In Situ Hybridization

1. Principles of in situ hybridization ; 2. The design and generation of probes for in situ hybridization ; 3. The preparation of non-radioisotopic hybridization probes ; 4. Principles and applications of complementary RNA probes ; 5. Quantification of in situ hybridization ; 6. Quantification of grains in in situ hybridization ; 7. Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization ; 8. Single and simultaneous nucleic acid detection in clinical samples ; 9. Combined in situ hybridization and immunocytochemistry: investigation of viral pathogenesis at the light and electron microscope levels ; 10. Super sensitive in situ DNA and RNA localisation