DNA-Protein Interactions
Springer-Verlag New York Inc.
978-1-0716-2846-1 (ISBN)
Authoritative and cutting-edge, DNA-Protein Interactions: Methods and Protocols aims to present genome-wide techniques that will complement the biochemistry-based protocols to aid researchers in their studies.
A DNA pull-down assay with diversity forms of competitor for detecting or evaluating protein-DNA interactions.- Enhanced yeast one-hybrid assays to study protein-DNA interactions.- Chromatin Immunoprecipitation on Fixed Tissues and Cell Lines.- The ChIP-exo method to identify genomic locations of DNA-binding Proteins at Near Single Base-Pair Resolution.- ChIP-seq Protocol for invVitro Cell Differentiation Systems.- Profiling Accessible Chromatin and Nucleosomes in the Mammalian Genome.- Dissecting Locus-Specific Chromatin Interactions by CRISPR Capture.- CUT&RUN to Profile Chromatin-Bound Proteins in Primary Mouse Neural Progenitor Cells.- Easy Hi-C: A Low Input Method for Capturing Genome Organization.- Single Molecule Imaging of DNA Protein Interactions Using DNA Curtains.- Quantifying the Binding and Target-Search Kinetics of Transcriptional Regulatory Factors by Live-Cell Single-Molecule Tracking.- Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles.- Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA.- Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering.- Mapping Transcription Regulation with Run-on and Sequencing Data Using The Web-Based tfTarget Gateway.- In embryo Gene Reporter Assays for Evaluation of Cis-Regulatory Regions.- In situ Hybridization as a Method to Examine Gene Regulatory Activity in vivo.- In situ Hybridization as a Method to Examine Gene Regulatory Activity in vivo.- Fishing for developmental regulatory regions: Zebrafish tissue-specific ATAC-seq.
Erscheinungsdatum | 30.11.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2599 |
Zusatzinfo | 54 Illustrations, color; 10 Illustrations, black and white; X, 284 p. 64 illus., 54 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Chemie | |
Schlagworte | ChRO-seq • Cut&Tag • Hi-ChIP • PRO-Seq • Protein Binding Arrays |
ISBN-10 | 1-0716-2846-1 / 1071628461 |
ISBN-13 | 978-1-0716-2846-1 / 9781071628461 |
Zustand | Neuware |
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