Plant Argonaute Proteins
Humana Press Inc. (Verlag)
978-1-4939-8404-6 (ISBN)
Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.
Plant ARGONAUTEs: Features, Functions and Unknowns.- Analysis of the Uridylation of Both ARGONAUTE–Bound MiRNAs and 5’ Cleavage Products of their Target RNAs in Plants.- In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts.- In vitro Analysis of ARGONAUTE–Mediated Target Cleavage and Translational Repression in Plants.- Establishment of an In Vivo ARGONAUTE Reporter System in Plants.- Immunoprecipitation and High–throughput Sequencing of ARGONAUTE–Bound RNAs from Plants.- Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing.- Dissecting the Sub–Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA–Directed DNA Methylation Pathway in Plants.- Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana.- Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair.- Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas.- ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation.- Cloning and Characterization of ARGONAUTE Genes in Tomato.- Non–Radioactive Detection of Small RNAs Using Digoxygenin–Labeled Probes.- Non–Radioactive Plant Small RNA Detection Using Biotin–Labeled Probes.- Computational Analysis of Genome–Wide ARGONAUTE–Dependent DNA.- Structural and Functional Characterization of Plant ARGONAUTE MID Domains.- Identification and Analysis of WG/GW ARGONAUTE–Binding Domains.- Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms.- Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES.
Erscheinungsdatum | 30.10.2018 |
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Reihe/Serie | Methods in Molecular Biology ; 1640 |
Zusatzinfo | 27 Illustrations, color; 20 Illustrations, black and white; XI, 297 p. 47 illus., 27 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
ISBN-10 | 1-4939-8404-7 / 1493984047 |
ISBN-13 | 978-1-4939-8404-6 / 9781493984046 |
Zustand | Neuware |
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