Plant Argonaute Proteins

Plant ARGONAUTEs: Features, Functions and Unknowns.- Analysis of the Uridylation of Both ARGONAUTE–Bound MiRNAs and 5’ Cleavage Products of their Target RNAs in Plants.- In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts.- In vitro Analysis of ARGONAUTE–Mediated Target Cleavage and Translational Repression in Plants.- Establishment of an In Vivo ARGONAUTE Reporter System in Plants.- Immunoprecipitation and High–throughput Sequencing of ARGONAUTE–Bound RNAs from Plants.- Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing.- Dissecting the Sub–Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA–Directed DNA Methylation Pathway in Plants.- Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana.- Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair.- Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas.- ARGONAUTE genes in Salvia miltiorrhiza: Identification, Characterization and Genetic Transformation.- Cloning and Characterization of ARGONAUTE Genes in Tomato.- Non–Radioactive Detection of Small RNAs Using Digoxygenin–Labeled Probes.- Non–Radioactive Plant Small RNA Detection Using Biotin–Labeled Probes.- Computational Analysis of Genome–Wide ARGONAUTE–Dependent DNA.- Structural and Functional Characterization of Plant ARGONAUTE MID Domains.- Identification and Analysis of WG/GW ARGONAUTE–Binding Domains.- Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms.- Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES.