Practical Enzymology
Seiten
2004
|
1., Auflage
Wiley-VCH (Verlag)
978-3-527-30444-8 (ISBN)
Wiley-VCH (Verlag)
978-3-527-30444-8 (ISBN)
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Providing a broad experimental, this book contains a theoretical introduction together with practical protocols, considering all aspects of enzymology. The fundamental experiments in enzymology are presented in very clear and easily realizable protocols, with each experiment accompanied by a section giving the theoretical background.
Essential reading for all scientists and practitioners working with enzymes.
Essential reading for all scientists and practitioners working with enzymes.
INTRODUCTION
ENZYME NOMENCLATURE
ENZYME REACTIONS
Theory of the enzyme reaction
Michaelis-Menten equation
Theory of the enzyme test
Theory of coupled enzyme reactions
Substrate determination
Instrumental aspects
Enzyme assays
Diverse methods
Enzyme immunoassays
BINDING MEASUREMENTS
Irreversible, reversible, specific and unspecific binding
Binding measurement by size discrimination
Spectroscopic techniques
Other methods for binding measurements
ENZYMES IN TECHNICAL APPLICATIONS
Principles of enzyme immobilisation
Methods for enzyme immobilisation
Analysis of immobilised enzymes
Enzyme reactors
Biosensors
Immobilized enzymes in therapy
APPENDIX
List of enzymes according to EC numbers
Sprache | englisch |
---|---|
Maße | 170 x 240 mm |
Gewicht | 523 g |
Einbandart | Paperback |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Schlagworte | Enzyme |
ISBN-10 | 3-527-30444-4 / 3527304444 |
ISBN-13 | 978-3-527-30444-8 / 9783527304448 |
Zustand | Neuware |
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