Molecular Toxicology Protocols

Analysis of DNA Adducts.- 32P-Postlabeling Analysis of DNA Adducts.- Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot.- DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry.- Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles.- Analysis of DNA Strand Cleavage at Abasic Sites.- Detection of Chromosomal and Genome-Wide Damage.- Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes.- Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk.- Flow Cytometric Analysis of Micronuclei in Erythrocytes.- The Comet Assay.- Computerized Image Analysis Software for the Comet Assay.- The Comet-FISH Technique.- DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis.- Detection and Characterization of Surrogate Gene Mutation.- Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes.- Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems.- Methods for Detecting Somatic Mutations In Vitro.- Molecular Analysis of Mutations in the Human HPRT Gene.- Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes.- The GPA In Vivo Somatic Mutation Assay.- Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR.- Detection and Characterization of Cancer Gene Mutation.- Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis.- Analysis of K-RAS and P53 Mutations in Sputum Samples.- Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution.- Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection.- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions.- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR.- Analysis of DNA Repair Mechanisms.- Microsatellite Instability.- Unscheduled DNA Synthesis.- Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation.- An Immunoassay for Measuring Repair of Ultraviolet Photoproducts.- Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells.- Measuring Recombination Proficiency in Mouse Embryonic Stem Cells.- Array Technologies.- Strategies for Measurement of Biotransformation Enzyme Gene Expression.- Genotyping Technologies.- TaqMan® Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material.- Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expression.- Apoptosis.- Quantification of Selective Phosphatidylserine Oxidation During Apoptosis.- Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Iron.- Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.

"This book should be of interest to both specialist researchers as well as members of the wider scientific community..." - British Journal of Anesthesiology