Molecular Toxicology Protocols

Part I. Analysis of DNA Adducts 32P-Postlabeling Analysis of DNA Adducts David H. Phillips, Alan Hewer, and Volker M. Arlt Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot Masako Ochiai, Takashi Sugimura, and Minako Nagao DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry Karen H. Dingley, Esther A. Ubick, John S. Vogel, and Kurt W. Haack Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles Iovanna Pandelova, Stephen R. Hewitt, and John B. Hays Analysis of DNA Strand Cleavage at Abasic Sites Walter A. Deutsch and Vijay Hegde Part II. Detection of Chromosomal and Genome-Wide Damage Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes Pataje G. S. Prasanna and William F. Blakely Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk Xifeng Wu, Yun-Ling Zheng, and T. C. Hsu Flow Cytometric Analysis of Micronuclei in Erythrocytes, Jan Grawe The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage Gunter Speit and Andreas Hartmann Computerized Image Analysis Software for the Comet Assay R. C. Chaubey The Comet-FISH Technique: A Tool for Detection of Specific DNA Damage and Repair Alexander Rapp, Michael Hausmann, and Karl Otto Greulich DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis Nina Joshi and Stephen G. Grant Part III. Detection and Characterization of Surrogate Gene Mutation Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems Roy R. Swiger Methods for Detecting Somatic Mutations In Vitro: The Human T-Cell Cloning Assay Selecting for HPRT Mutants Sai-Mei Hou Molecular Analysis of Mutations in the Human HPRT Gene Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes James C. Fuscoe The GPA In Vivo Somatic Mutation Assay Stephen G. Grant Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR: Detecting Somatic Mutations in Humans and Mice Seishi Kyoizumi, Yoichiro Kusunoki, and Tomonori Hayashi Part IV. Detection and Characterization of Cancer Gene Mutation Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis Therese Sorlie, Hilde Johnsen, Phuong Vu, Guro Elisabeth Lind, Ragnhild Lothe, and Anne-Lise Borresen-Dale Analysis of K-RAS and P53 Mutations in Sputum Samples Weimin Gao and Phouthone Keohavong Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution Barbara L. Parsons, Page B. McKinzie, and Robert H. Heflich Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and Solutions Vibhuti Gupta, Reetakshi Arora, Anand Ranjan, Narendra K. Bairwa, Dheeraj K. Malhotra, P. T. Udhayasuriyan, Anjana Saha, and Ramesh Bamezai Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions Toshinari Minamoto Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR Michael J. Villalobos, Christopher J. Betti, and Andrew T. M. Vaughan Part V. Analysis of DNA Repair Mechanisms Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery Anjana Saha, Narendra K. Bairwa, and Ramesh Bamezai Unscheduled DNA Synthesis: A Functional Assay for Global Genomic Nucleotide Excision Repair Crystal M. Kelly and Jean J. Latimer Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation Jennifer M. Johnson and Jean J. Latimer An Immunoassay for Measuring Repair of Ultraviolet Photoproducts Shirley McCready Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells Petra Pfeiffer, Elke Feldmann, Andrea Odersky, Steffi Kuhfittig-Kulle, and Wolfgang Goedecke Measuring Recombination Proficiency in Mouse Embryonic Stem Cells Andrew J. Pierce and Maria Jasin Part VI. Array Technologies Strategies for Measurement of Biotransformation Enzyme Gene Expression Marjorie Romkes and Shama C. Buch Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening Marjorie Romkes and Shama C. Buch TaqMantm Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material Kaori Shintani-Ishida, Bao-Li Zhu, and Hitoshi Maeda Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expression Tony E. Godfrey and Lori A. Kelly Part VII. Apoptosis Quantification of Selective Phosphatidylserine Oxidation During Apoptosis James P. Fabisiak, Yulia Y. Tyurina, Vladimir A. Tyurin, and Valerian E. Kagan Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Iron James P. Fabisiak, Grigory G. Borisenko, and Valerian E. Kagan Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay Michael E. Gehring and Patrick P. Koty Index