Molecular Toxicology Protocols

Array-Based Immunoassays with Rolling-Circle Amplification Detection.- Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis.- Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage.- Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System.- Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques.- Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).- Strategies for Measurement of Biotransformation Enzyme Gene Expression.- Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening.- TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material.- 32P-Postlabelling Analysis of DNA Adducts.- Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot.- DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry.- Analysis of DNA Strand Cleavage at Abasic Sites.- Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes.- Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk.- Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair.- Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice.- The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay.- Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice.- Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes.- Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems.- The Human T-CellCloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation.- Molecular Analysis of HPRT Mutations in Human Lymphocytes.- Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes.- Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis.- Detection of Point Mutations of K-ras Oncogene and p53 Tumor Suppressor Gene in Sputum Samples.- ACB-PCR Quantification of Somatic Oncomutations.- Gel-Based Nonradioactive Single Strand Conformational Polymorphism and Mutation Detection.- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesion.- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR.- Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells.- The Sister Chromatid Exchange (SCE) Assay.- The Gene Cluster Instability (GCI) Assay for Recombination.- Measuring Recombination Proficiency in Mouse Embryonic Stem Cells.- Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery.- Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic Nucleotide Excision Repair.- An Immunoassay for Measuring Repair of UV Photoproducts.- Analysis of Double-Strand Break Repair by Non-Homologous DNA End Joining in Cell-Free Extracts from Mammalian Cells.- Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF42 Extracellular Flux Analyzer and a Luciferase ATP Assay.- Quantification of Selective Phosphatidylserine Oxidation During Apoptosis.- Quantitative Method of Measuring Phophatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron.- Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.