Handbook of Vascular Biology Techniques

Preface.- Section 1 In vitro techniques.-  In vitro angiogenesis assay - endothelial migration, proliferation, and tube formation.-  Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis.- Induction of hypoxia in vascular endothelial cell culture.- Evaluating In Vitro Angiogenesis using Live Cell Imaging.- Isolation of Endothelial Progenitor Cells (EPCs).- Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis.- Assessment of vascular function and contractility, ex vivo.- Measurement of Aβ uptake by cerebrovascular smooth muscle cells.-  Measurement of Intracellular Ca2+ in Human Endothelial cells.- Section 2 In vivo and ex vivo manipulations.-  Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion.-  Sponge implant model of inflammatory angiogenesis.- The chick embryo chorioallantoic membrane assay.- Dorsal air sac assay.- Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals.- Hypoxia-induced retinal angiogenesis in adult zebrafish.- Angiogenesis in regenerating adult zebrafish tail fin.- Methods for studying developmental angiogenesis in zebrafish.- Isolation and Expansion of Brain Microvascular Endothelial Cells.- Section 3 Imaging and histological analysis.- Adipose angiogenesis.- Assessing tumor angiogenesis in histological samples.- Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the  embryonic mouse skin and adult mouse cornea.- Computed Tomography Angiography: Fundamental techniques and data interpretation.- Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation.- Single-Photon Emission Tomography of the brain in vascular pathology.- Section 4 Miscellaneous novel techniques in vascular biology.- Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues.- Preparation of liposomes withdual fluorophores to follow real-time content release in vivo.- Vascular Flow Modelling using Computational Fluid Dynamics.- Reverse Transcription Real-Time PCR Protocol for gene expression analyses.- Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis.- Genomic microarray analysis.- Selection of appropriate housekeeping genes for quality control.- Endothelial transcriptomic analysis.- Protocol for Multiplex Amplicon Sequencing using Barcoded Primers.- Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells:  Identification and Quantification.- A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood.- Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry.