Rice
Springer-Verlag New York Inc.
978-1-0716-4203-0 (ISBN)
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Authoritative and cutting-edge, Rice: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Hydroponic culture of rice seedlings for stress response assay.- Total RNA extraction from rice vegetative tissues using magnetic beads.- Total RNA extraction from starchy rice endosperm.- RNA extraction from rice immature embryo using laser capture microdissection.- Using immunoblotting for detecting of a circadian clock component, Os−GI, in rice leaves.- Preparation and Sectioning of Paraffin-Embedded Tissue for Histology and Histochemistry.- Spatial Analysis of Gene Expression by In Situ Hybridization.- Purification of Anti-OSH1 IgG and Immunohistochemical Staining of Rice Shoot Apical Meristem.- Agrobacterium-mediated transformation and targeted mutagenesis using SpCas12f in rice.- Gene targeting via CRISPR/Cas9-mediated DNA double-strand break induction in rice.- Precise base substitution using CRISPR/Cas-mediated base editor in rice.- DeLTa-seq: High-throughput targeted RNA-seq of rice leaves without RNA purification.- Lasy-seq: a high-throughput 3′ RNA-seq method for large-scale transcriptome profiling in rice.- Profiling of 24nt phasiRNAs in rice anther by massively parallel sequencing and stem-loop reverse transcription PCR.- Expression and purification of pAG-MNase for CUT&RUN.- Chromatin immunoprecipitation from shoot apices and young panicles of rice.- In vitro Genome-wide identification of transcription factor binding sites.- High-efficient transient gene transfection for transcriptional activity assay using rice protoplasts on large-scale platform.
Erscheint lt. Verlag | 4.1.2025 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 34 Illustrations, color; 13 Illustrations, black and white; CLX, 160 p. 10 illus. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Technik ► Umwelttechnik / Biotechnologie | |
Weitere Fachgebiete ► Land- / Forstwirtschaft / Fischerei | |
ISBN-10 | 1-0716-4203-0 / 1071642030 |
ISBN-13 | 978-1-0716-4203-0 / 9781071642030 |
Zustand | Neuware |
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