Lotus japonicus Handbook

Lotus japonicus’s a model system.- Lotus-related species and their agronomic importance.- Drought and saline stress.- Methods for studying nodule development and function.- A procedure for in vitro nodulation studies.- Arbuscular mycorrhiza.- Concurrent visualization of gusA and lacZ reporter gene expression.- Embedding root and nodule tissue in plastic (BMM).- RNA isolation using phase extraction and LiCl precipitation.- RNA isolation using CsCl gradients.- 96-Well DNA isolation method.- Genome structure analysis.- Transcriptome analysis using cDNA arrays.- Lotus japonicus expression database.- Procedures for mass spectrometric proteome analysis.- Isolation of peribacteroid membranes for proteome analysis.- Metabolome analysis using GC-MS.- Mutagenesis.- A mutant catalogue of Lotus japonicus.- Tilling.- Ds gene-tagging.- Mapping and map-based cloning.- An in silico strategy towards the development of legume genome anchor markers using comparative sequence analysis.- Information transfer: mapping and cloning in other legumes.- Agrobacterium-mediated in vitro transformation.- Induction of hairy roots for symbiotic gene expression studies.- Transformation-regeneration procedure for Lotus japonicus.- Agrobacterium rhizogenes pRi TL-DNA integration system: a gene vector for Lotus japonicus transformation.- Vectors for reverse genetics and expression analysis.- Nitrate assimilation: Influence of nitrogen supply.- Nitrate and ammonium assimilatory enzymes.- Transgenic plants affected in nitrate assimilation.- Secondary metabolite profiling.- Phenolic Compounds: extraction and analysis.- Elicitation of isoflavan phytoalexins.- An update of work published on Lotus japonicus.