Foodborne Bacterial Pathogens

 Direct or DNA extraction-free amplification and quantification of foodborne pathogens.- The use of multiplex real time PCR for the simultaneous detection of foodborne bacterial pathogens.- Viability detection of foodborne bacterial pathogens in food environment by PMA-qPCR and by microscopy observation.- Electrochemical Detection of Nucleic Acids using 3D Graphene Screen Printed Electrodes.- Application of MinION long read sequencer for semi-targeted detection of foodborne pathogens.- Integrating MALDI-TOF mass spectrometry with machine learning techniques for rapid antimicrobial resistances screening of foodborne bacterial pathogens.- Assessment of viability of Listeria monocytogenes by flow cytometry. - Method to study the survival abilities of foodborne bacterial pathogens under food processing conditions.- Characterization of bacterial membrane fatty acid profiles for biofilm cells.- Comparing gene expression between planktonic and biofilm cells of foodborne bacterial pathogens through RT-qPC,- Characterization of Food-Borne Pathogens in Biofilms: A 4D Structural Dynamics Approach Using HCS-CLSM.- Zebrafish larvae microinjection and automated fluorescence microscopy for studying Klebsiella pneumoniae infection and the host immune response.- Flow Cytometry and Automated Microscopy Integration for Phagocytic Analysis of hypervirulent Klebsiella pneumoniae in Dictyostelium discoideum.- A Comprehensive Guide to Quality Assessment and Data Submission for Genomic Surveillance of Enteric Pathogens.- Genomic pipeline for analysis of mutational events in bacteria.- Using GWAS and Machine Learning to identify and predict genetic variants associated with foodborne bacteria phenotypic traits.- Identifying putative biomarkers of foodborne pathogens using a metabolomic approach.- Illumina MiSeq 16S rRNA Gene Library Preparation for Poultry Processing Microbiome Analyses.- Bioinformatic pipeline for profiling foodborne bacterial ecology and resistome from short-read metagenomics.