Effect of the singular cell age on Saccharomyces pastorianus ssp. carlsbergensis proliferation

Aging in yeast cells is understood as a decrease in physiological function, particularly in their ability to adapt to environmental changes. Saccharomyces yeasts are used in industrial processes, such as the production of bioethanol, beer, and wine. We lack rapid, non-invasive methods to analyse the impact of age on process performance in real-time at the single-cell level in high-throughput. This thesis examined chitin-containing bud scars in yeasts as a surface marker to determine the single-cell age within populations. These scars were used to track cell age during processes and for population separation based on age.