Biosafety Assessment of Probiotic Potential

Determination of biogenic Amine Production.- Determination of Gelatinases,Glycosidases, and Enolase production.- Determination of β-Glucuronidase Production.- Determination of Nitroreductase Production.- Determination of Azoreductase production.- Determination of Hemolytic activity.- Determination of DNAse Activity.- Determination of Bile Salts Deconjugation.- Determination of D-lactic Acid Production.- Determination of Antibiotic Resistance.- Determination of Antibiotic Resistance Gene Transfer.- Determination of Toxin Production.- Detection of Toxin Genes by PCR Based Methods.- Determination of Presence of Plasmids and Virulence and Structural Genes.- Determination of Toxicity through Cytotoxicity Assays.- In vitro Evaluation of the Nitric Oxide Pathway.- Assessment of Capsule Formation.- Assessment of Platelet aggregation.- Assessment of Fibrinogen and Fibronectin Binding Activity.- Assessment of ProbioticsAdhesion to Mammalian Cells.- Assessment of Mutagenicity.- Assessment of Induction and Destruction of Thrombi.- Assessment of degradation of mucin.- Evaluation of General Health Status of the Animals during the in-Life Phase.- Assessment of Bacterial Translocation Through Blood Cultures.- Determination of Splenic weight index and Weight-to-length ratio.- Determination of Total liver glutathione and plasma malondialdehyde concentrations.- Determination of Serum Lactate and Fecal Calprotectin for Assessing The Intestinal Inflammation.- In vivo Evaluation of Adhesion properties of Probiotics.- Determination of Streptomyces Probiotics oral administration in broiler chicken.- Determination of infectivity of probiotics using animal models.- Determination of Infectivity of Probiotics using Immunosuppressed Hosts.- Assessment of Reproductive toxicity.- Assessment of Developmental toxicity in Zebrafish model.- Assessment of Inflammation in Animal Models (Macroscopic Or Histological Inflammation In The Ileum Or In The Colon).- Assessment of inflammation in animal models (Quantification of TNFA, IFNG, IL4, and IL10 mRNAs by Real-time PCR).- Assessment of inflammation in animal models (Quantification of TNF-α, IFN-γ, IL-4, & IL-10 proteins by ELISA).- Detection of Myeloperoxidase activity by Enzyme Linked Immunosorbent Assay.- Assessment of Bacterial Translocation Through Mesenteric Lymph Nodes [MLN] and Spleen Cultures.