Plant Synthetic Biology

Heterologous production of plant terpenes in the photosynthetic bacterium Rhodobacter capsulatus.- Design of multiplexing CRISPR/Cas9 constructs for plant genome engineering using the GoldenBraid DNA assembly standard.- pSHDY - a new tool for genetic engineering of cyanobacteria.- Cas9-mediated targeted mutagenesis in plants.- Generation of stable, light-driven co-cultures of cyanobacteria with heterotrophic microbes.- Gene editing in green alga Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins.- Coexpression and reconstitution of enzymatic cascades in bacteria using UbiGate.- Homo-FRET imaging to study protein-protein interaction and complex formation in plants.- Plant X-tender toolbox for the assembly and expression of multiple transcriptional units in plants.- AGROBEST: a highly efficient Agrobacterium-mediated transient expression system in Arabidopsis seedlings.- Genetically-encoded biosensors for the quantitative analysis of auxin dynamics in plant cells.- Exploiting the Gal4/UAS system as plant orthogonal molecular toolbox to control reporter expression in Arabidopsis protoplasts.- Mathematical Modelling in Plant Synthetic Biology.- In vivo Epitope-tagging of Plant Mitochondria.- Engineering destabilizing N‑termini in plastids.- Trichome transcripts as efficiency control for Synthetic Biology and Molecular Farming.