Clinical Applications of Nucleic Acid Amplification

A Brief Practical Guide to PCR.- Managing a PCR Contamination Event in a Molecular Pathology Laboratory.- Next Generation Sequencing Informatic Architecture Considerations.- Nuclease Enrichment and qPCR Detection of Rare Nucleotide Variants.- MYD88 L265P Mutation Detection by ddPCR: Recommendations for Screening and Minimal Residual Disease Monitoring.- Capture Methylation-Sensitive Restriction Enzyme Sequencing (Capture MRE-Seq) for Methylation Analysis of Highly Degraded DNA.- LT-RPA: An Isothermal DNA Amplification Approach for Improved Microsatellite Genotyping and Microsatellite Instability Detection.- Liquid Biopsy for Advanced Cancer: An Amplicon Based Massively Parallel Sequencing Panel Approach to Precision Oncology.- Mate Pair Sequencing: Next Generation Sequencing for Structural Variant Detection.- Cell Subset-Specific Chimerism Testing by Short Tandem Repeats Analysis for Engraftment Monitoring After Hematopoietic Stem Cell Transplantation.- Immune Cell Lineage-Specific Chimerism Testing by Next-Generation Sequencing for Engraftment Monitoring After Allogeneic Hematopoietic Stem Cell Transplantation.- Rapid Whole Genome Sequencing for Diagnosis of Single Locus Genetic Diseases in Critically Ill Children.- EOSAL-CNV for Easy and Rapid Detection of CNVs by Fragment Analysis.- Rapid Cycle and Extreme Polymerase Chain Reaction.- CRISPR-Cas12-Based Diagnostic Applications in Infectious and Zoonotic Diseases.- SARS-CoV-2 Sequencing for Variant Surveillance.- Tuberculosis Diagnosis Using Isothermal Nucleic Acid Amplification in a Paper-and-Plastic Device.- Loop-Mediated Isothermal Amplification on Paper Microfluidic Chips for Highly Sensitive and Specific Zika Virus Detection Using Smartphone.- Ultrasensitive Reverse Transcriptase Loop-Mediated Isothermal Amplification (US-LAMP) Based Detection of Malaria Infection from Dried Blood Spots.