Cartilage Tissue Engineering

Cartilage Tissue Engineering: An Introduction.- Chondrocyte Isolation and Expansion.- Isolation of Chondrons from Hyaline Cartilage.- Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation.- Physioxic Culture of Chondrogenic Cells.- Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.- Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation.- Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells.- The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans.- Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy.- Metabolomic Profiling to Understand Chondrocyte Metabolism.- Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes.- Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads.- Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis.- Modulation of MicroRNA Expression during In Vitro Chondrogenesis.- Purification and Isolation of Proteins from Hyaline Cartilage.- Histological Preparation and Evaluation of Cartilage Specimens.- Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology.- Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization.- Viral Gene Delivery in Chondrocytes.- Decellularization of Articular Cartilage: A Hydrochloric Acid-Based Strategy.- Self-Assembly Culture Model for Engineering Musculoskeletal Tissues.- Osteochondral Explants Isolation and Culture under a Compression and Shear Bioreactor.- A Mouse Model of Acute Cartilage Injury and Repair.- Small-Animal Compression Models of Osteoarthritis.- Modelling and Assessing Osteoarthritis in Mice by Destabilization of the Medial Meniscus (DMM).- Collagenase-Induced Osteoarthritis in Mice.- Platelet-Rich Plasma for Intra-Articular Injections: Preclinical and Clinical Evidence.