Listeria Monocytogenes

Traditional Methods of Analysis for Listeria monocytogenes.- MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes.- Sample Preparation for qPCR Detection of Listeria from Food.- qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay.- Serotype Assignment by Sero-Agglutination, ELISA, and PCR.- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes.- Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua.- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray.- High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format.- Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities.- Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis.- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes.- Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the “SOEing” Method.- Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes.- Internalization Assays for Listeria monocytogenes.- Microscopy of Intracellular Listeria monocytogenes in Epithelial Cells.- Control of Listeria monocytogenes Biofilms in a Simulated Food Processing Environment.- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing (FP).- Isolation and Evaluation of Anti-Listeria Lactococcus lactis from Vegetal Sources.