Next Generation Culture Platforms for Reliable In Vitro Models

Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State.- Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids.- Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation.- A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors.- Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres.- Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models.- Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues.- Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models.- Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold.- A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells.- 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids.- Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics.- Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC).- Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columns.- Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer.- Isolation, Characterization, and MicroRNAs Analysis of Extracellular Vesicles from Bovine Oviduct and Uterine Fluids.- Biomimetic 3D-Bone Tissue Model.- Using the Air-Liquid Interface Approach to Foster Apical-Basal Polarization of Mammalian FemaleReproductive Tract Epithelia In Vitro.- Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa.- Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro.