Fundamentals of Enzyme Engineering

Prof. Yoo, Young Je is currently a Professor at the School of Chemical Engineering, Seoul National University and holds a B.S. from Department of Chemical Engineering, Seoul National University and a Ph.D. at the Department of Chemical Engineering, University of Maryland. His research interests are understanding of enzyme’s function at the molecular level and engineering and design of enzyme activity, stability and specificity, and enzymatic reactions for useful chemicals production and environmental applications. Prof. Feng, Yan is currently a Professor at the School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, China and holds B.S. from Department of Molecular Biology, Jilin University, M.S.from State Key Laboratory of Enzyme Engineering,and M.D. from Basic Medical Sciences School, Bethune Medical University. Her research interests focus on investigating the enzyme structure-function relationship, as well as employing rational design and direct evolution to engineer useful biocatalysts. Prof. Kim, Yong Hwan is currently a Professor at the School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology and holds a B.S., M.S., and Ph.D. from Department of Chemical Engineering, Seoul National University. He is actively developing new biocatalysts for the conversion of C1 gas compounds to value-added chemicals based on the profound experience in industry and national research institutes. He has been involved in the commercialisation of enzyme-catalyzed polymerization of phenolics for special coating materials and enzyme-catalyzed production of linin based material for automotive industry. Prof. Yagonia, Camila Flor J. is currently a professor at the Department of Chemical Engineering, San Carlos University, Philippines. She got BS and Ms degrees from San Carlos University and PhD from Seoul National University. Her research interests include bioenergy production, production of chemicals and materials using biotechnology, and industrial applications of enzymes.

I. Introduction to Enzyme Engineering.- 1. Introduction.- 2. Biosynthesis of Enzymes.- 3. Production of Enzymes.- II. Enzyme Reaction Engineering.- 4. Enzyme Reaction Kinetics.- 5. Regeneration of Cofactors.- 6. Immobilized Enzymes.- 7. Enzymes in Non-conventional Media.- III. Molecular Understanding of Enzymes.- 8. Engineering Tools for Enzymes.- 9. Enzyme Catalysis.- 10. Specificity of Enzymes.- 11. Thermodynamics and Stability.- IV. Application of Enzymes.- 12. Enzymes for Chemicals and Polymers.- 13. Enzymes for Food and Energy.- 14. Enzymes for Biosensors.- 15. Enzymes for Environment.