Mass Spectrometry of Protein Interactions (eBook)

Kevin M. Downard, PhD, is an Associate Professor in the School of Molecular and Microbial Biosciences at the University of Sydney in Australia. He has twenty years of research experience in mass spectrometry, with the past fifteen devoted to protein analysis.

Preface.

Contributors.

Chapter 1. Direct Characterization of Protein Complexes by
Electrospray Ionization Mass Spectrometry and Ion Mobility Analysis
(Joseph A. Loo and Catherine S. Kaddis).

1.1 Introduction.

1.2 Critical Aspects of the Experimental Procedure.

1.3 Solution Phase Equilibria and Gas Phase Dissociation.

1.4 Conclusions.

Chapter 2. Softly, Softly-Detection of Protein Complexes by
Matrix-Assisted Laser Desorption Ionisation Mass Spectrometry
(Kevin M. Downard).

2.1 Introduction.

2.2 First Glimpses and the First-Shot Phenomenon.

2.3 Matrix and Solution Criteria to Preserve Protein
Complexes.

2.4 Laser Fluence, Wavelength and Ion Extraction.

2.5 Preservation of Protein Complexes on Conventional Maldi
Targets.

2.6 Affinity Targets and Surfaces Coupled To Maldi.

2.7 Conclusions.

Chapter 3. Probing Protein Interactions Using
Hydrogen-Deuterium Exchange Mass Spectrometry (David D. Weis, Suma
Kaveti, Yan Wu and John R. Engen).

3.1 Introduction.

3.2 Hydrogen Exchange Background.

3.3 General HX-MS Method.

3.4 Interactions of Proteins.

3.5 Examples.

3.6 Conclusions.

Chapter 4. Limited Proteolysis Mass Spectrometry of Protein
Complexes (Maria Monti and Piero Pucci).

4.1 Introduction.

4.2 Limited Proteolysis Analysis.

4.3 Experimental Design.

4.4 Probing Protein-Protein Interactions.

4.5 Probing Protein-Nucleic Acid Interactions.

4.6 Protein-Ligand Interactions.

4.7 Probing Amyloid Fibril Core.

4.8 Conlusions.

Chapter 5. Chemical Cross-Linking and Mass Spectrometry for
Investigation of Protein-Protein Interactions (Andrea
Sinz).

Introduction.

5.1 Cross-Linking Strategies.

5.2 Functional Groups of Cross-Linking Reagents.

5.3 Mass Spectrometric Analysis of Cross-Linked Products.

5.4 Identification of Cross-Linked Produc.

5.5 Computer Software for Data Analysis.

5.6 Conclusions and Perspectives.

Chapter 6. Genesis & Application of Radical Probe Mass
Spectrometry (RP-MS) to Study Protein Interactions (Simin D.
Maleknia and Kevin M. Downard).

6.1 Genesis of Radical Probe Mass Spectrometry.

6.2 The Reactive Residue Side Chains.

6.3 Conditions Important to Radical Probe Mass Spectrometry
Experiments.

6.4 Generation of Radicals on Millisecond Timescales.

6.5 Applications of RP-MS to Studies of Protein
Interactions.

6.6 Onset of Oxidative Damage and Its Implications for Protein
Interactions.

6.7 Application of Radical Oxidation to Study Protein
Assemblies.

6.8 Modeling Protein Complexes with Data from RP-MS
Experiments.

6.9 Conclusions.

Index.