Molecular and Diagnostic Procedures in Mycoplasmology (eBook)

Front Cover 1
Molecular and Diagnostic Procedures in Mycoplasmology 4
Copyright Page 5
Contents 6
Contributors 12
Preface 16
Contents of Volume I 18
Mollicute–Host Interrelationships: Current Concepts and Diagnostic Implications 22
Section A: Diagnostic Genetic Probes 44
Chapter A1. Introductory Remarks 46
Chapter A2. Oligonucleotide Probes Complementary to 16S rRNA 50
Chapter A3. Cloned Genomic DNA Fragments as Probes 68
Chapter A4. PCR: Selection of Target Sequences 74
Chapter A5. PCR: Preparation of DNA from Clinical Specimens 82
Chapter A6. PCR: Amplification and Identification of Products 86
Chapter A7. PCR: Application of Nested PCR to Detection of Mycoplasmas 96
Chapter A8. PCR: Random Amplified Polymorphic DNA Fingerprinting 102
Section B: Immunological Tools 108
Chapter B1. Introductory Remarks 110
Chapter B2. ELISA in Small Animal Hosts, Rodents, and Birds 114
Chapter B3. ELISA in Large Animals 126
Chapter B4. ELISA in Human Urogenital Infections and AIDS 136
Chapter B5. ELISA in Respiratory Infections of Humans 144
Chapter B6. Monoclonal Antibodies as Diagnostic Tools 158
Chapter B7. Microimmunofluorescence 168
Chapter B8. Immunoblots and Immunobinding 172
Chapter B9. Differentiation of Mycoplasma genitalium from Mycoplasma pneumoniae by Immunofluorescence 190
Section C: Antibiotic Sensitivity Testing 200
Chapter C1. Introductory Remarks 202
Chapter C2. Problems and Opportunities in Susceptibility Testing of Mollicutes 206
Chapter C3. Determination of Minimal Inhibitory Concentration 210
Chapter C4. Cidal Activity Testing 220
Section D: Diagnosis of Specific Diseases 226
Chapter D1. Introductory Remarks 228
Chapter D2. Laboratory Diagnosis of Mycoplasma pneumoniae Infection 232
Chapter D3. Diagnosis of Sexually Transmitted Diseases 246
Chapter D4. Diagnosis of Neonatal Infections 258
Chapter D5. Mycoplasmas in AIDS Patients 268
Chapter D6. Mycoplasma Infections of Cattle 276
Chapter D7. Mycoplasma Infections of Goats and Sheep 286
Chapter D8. Mycoplasma Infections of Swine 296
Chapter D9. Mycoplasma Infections of Poultry 304
Chapter D10. Diagnosis of Spiroplasma Infections in Plants and Insects 314
Chapter D11. Detection of Phytoplasma Infections in Plants 320
Chapter D12. Identification of Mollicutes from Insects 334
Section E: Experimental Infections 344
Chapter E1. Introductory Remarks 346
Chapter E2. Experimental Mycoplasmal Respiratory Infections in Rodents 348
Chapter E3. Urogenital Infections in Rodents 358
Chapter E4. Experimental Models of Arthritis 370
Chapter E5. Experimental Infections in Poultry 382
Chapter E6. Experimental Infections of Swine 392
Chapter E7. Experimental Infections in Cattle 398
Chapter E8. Experimental Infections of Plants by Spiroplasmas 406
Chapter E9. Experimental Phytoplasma Infections in Plants and Insects 412
Chapter E10. Mycoplasmas and in Vitro Infections of Cell Cultures with HIV 420
Section F: Diagnosis of Mycoplasma Infections of Cell Cultures 426
Chapter F1. Introductory Remarks 428
Chapter F2. Isolation of Mycoplasmas from Cell Cultures by Axenic Cultivation Techniques 432
Chapter F3. Detection of Mycoplasmas by DNA Staining and Fluorescent Antibody Methodology 440
Chapter F4. Detection of Mycoplasma Infection by PCR 452
Chapter F5. Antibiotic Treatment of Mycoplasma-lnfected Cell Cultures 460
Chapter F6. Prevention and Control of Mycoplasma Infection of Cell Cultures 466
Appendix 474
Table I. Genus Mycoplasma and Major Characteristics 476
Table II. Genera Ureaplasma and Acholeplasma 479
Table III. Genera Anaeroplasma and Asteroleplasma 479
Table IV. Genera Entomoplasma and Mesoplasma 480
Table V. Group Classification of Genus Spiroplasma 481
Index 484
Color Plate Section 488