Plant Tissue Culture Concepts and Laboratory Exercises

Overview and Scheduling of Laboratory Exercises
HISTORY OF PLANT TISSUE CULTURE
History of Plant Tissue and Cell Culture, J.D. Caponetti, D.J. Gray, and R.N. Trigiano

SUPPORTING METHODOLOGIES
General Laboratory Requirements, Media, and Sterilization Methods, E.G. Sutter
Nutrition of Callus Cultures, J.D. Caponetti
Histological Techniques, R.N. Trigiano, K.R. Malueg, and E.T. Graham
Photographic Methods for Plant Cell and Tissue Culture, D.J. Gray
Statistical Analysis of Plant Tissue Culture Data, M.E. Compton

PROPAGATION TECHNIQUES
Propagation from Preexisting Meristems, M.E. Kane
Micropropagation of Syngonium by Shoot Culture, M.E. Kane
Micropropagation of Potato by Node Culture and Microtuber Production, M.E. Kane
Micropropagation of Lilacs, D.D. McCown and A.J. Daun
Propagation from Nonmeristematic Tissues-Organogenesis, O.J. Schwarz and R.M. Beaty
Direct Shoot Organogenesis from Leaf Explants of Chrysanthemum, R.N. Trigiano and R.A. May
Shoot Organogenesis from Cotyledon Explants of Watermelon, M.E. Compton and D.J. Gray
Direct and Indirect Shoot Organogenesis from Leaves of Torenia fournieri, M.P. Bridgen
Shoot Organogenesis from Petunia Leaves, J.E. Preece
Nonzygotic Embryogenesis, D.J. Gray
Embryogenic Callus and Suspension Cultures from Leaves of Orchardgrass, D.J. Gray, R.N. Trigiano, and B.V. Conger
Somatic Embryogenesis from Seeds of Melon, D.J. Gray
Somatic Embryogenesis from Mature Peanut Seed, H.Y. Wetzstein and C. Baker
Direct Somatic Embryogenesis from Leaves and Flower Receptacles of Cineraria, R.N. Trigiano, M.C. Scott, and K.R. Malueg
Somatic Embryogenesis from Immature Seeds of Yellow Poplar, S.A. Merkle
Induction of Somatic Embryogenesis in Conifers, S.M. Attree, P.J. Rennie, and L.C. Fowke

CROP IMPROVEMENT TECHNIQUES
Use of Protoplasts for Plant Improvement, M.E. Compton, J.A. Saunders, and R. Veilleux
Isolation, Culture and Fusion of Tobacco and Potato Protoplasts, R.E. Veilleux and M.E. Compton
Isolation of Protoplasts from Leaves of Chrysanthemum and Orchardgrass, R.N. Trigiano
Isolation and Culture of Protoplasts from Embryogenic Yellow Poplar Suspension Cultures, S.A. Merkle
Haploid Cultures, S.M. Reed
Production of Haploid Tobacco Plants Using Anther Culture, S.M. Reed
Transformation, M. López-Meyer, I.E. Maldonado-Mendoza, and C.L. Nessler
Transformation of Tobacco and Carrot Using Agrobacterium tumefaciens and Expression of the ß-Glucuronidase (GUS) Reporter Gene, I.E. Maldonado-Mendoza, M. López-Meyer, and C.L. Nessler
Transformation of Chrysanthemum Leaf Explants Using Agrobacterium tumefaciens, R.N. Trigiano
Construction and Use of a Simple Gene Gun for Particle Bombardment, D.J. Gray, E. Hiebert, C.-M. Lin, M.E. Compton, and V.P. Gaba
Germplasm Preservation, L.E. Towill
Vitrification as a Method to Cryopreserve Shoot Tips, L.E. Towill
Secondary Product Expression in Vitro, M.A.L. Smith
Pigment Production in Ajuga Cell Cultures, M.A.L. Smith
In Vitro Plant Pathology, C.J. Baker, N.M. Mock, and E.W. Orlandi
SPECIAL APPLICATIONS
Commercial Laboratory Production, G.R.L. Suttle
Indexing for Plant Pathogens, M.J. Klopmeyer
Appendix: List of Suppliers
Index