Atlas of Human Tumor Cell Lines (eBook)

Front Cover 1
Atlas of Human Tumor Cell Lines 4
Copyright Page 5
Table of Contents 6
Preface 12
Chapter 1. Quality Control and Characterization of Cell Lines 14
I. Introduction 14
II. Seed Stock Concept 15
III. Microbial Contamination 16
IV. Cellular Cross-Contamination 21
V. Origin and Function 26
VI. Conclusions 27
References 27
Chapter 2. Human Glioma Cell Lines 30
I. Introduction 30
II. Cell Lines Derived from Glioblastoma Multiforme and Anaplastic Astrocytoma 31
III. Cell Lines Derived from Oligodendroglioma 44
IV. Cell Lines Derived from Primitive Neuroectodermal Tumor/Medulloblastoma 45
V. Concluding Remarks 46
References 47
Chapter 3. Tumor Cell Lines of the Peripheral Nervous System 56
I. Neuroblastoma 58
II. Peripheral Neuroepithelioma 74
Acknowledgments 83
References 83
Chapter 4. Head and Neck Tumor Cell Lines 92
I. Introduction 92
II. Methods of Establishment and Maintenance 94
III. Morphology 109
IV. Other Characteristics 126
V. Discussion 128
VI. Future Prospects 130
References 130
Chapter 5. Cell Culture of Lung Cancers 134
I. Cellular Origins of Lung Cancers 134
II. Major Forms of Lung Cancer 136
III. Defined Media for the Culture of Lung Tumors 137
IV. Establishment of Small-Cell Lung Carcinoma Cell Lines 138
V. Other Neuroendocrine Tumors 147
VI. Cell Lines Derived from Non-Small-Cell Lung Carcinoma 149
VII. Mesotheliomas 159
VIII. Conclusions 159
References 159
Chapter 6. Cell Lines from Human Breast 174
I. Introduction 174
II. Methods of Establishment and Maintenance 175
III. Morphology 175
IV. Discussion 193
V. Future Prospects 194
Acknowledgment 194
References 194
Chapter 7. Hepatocellular Carcinomas 198
I. Introduction 198
II. Undifferentiated Hepatocellular Carcinoma Cell Line HLE 199
III. Differentiated Hepatocellular Carcinoma Cell Lines 202
IV. Differentiated Hepatoblastoma Cell Lines 212
V. Conclusions 218
References 218
Chapter 8. Hematopoietic Cell Lines 226
I. Introduction 226
II. Culture of Hematopoietic Cells 227
III. Leukemia–Lymphoma Cell Lines 230
IV. Characterization of Leukemia Cell Lines 232
V. Growth Factor-Dependent Leukemia Cell Lines 255
VI. Hodgkin's Disease-Derived Cell Lines 256
VII. Future Prospects 257
VIII. Lists of Leukemia Cell Lines 262
References 262
Chapter 9. Human Sarcoma Cells in Culture 264
I. Introduction 264
II. Methods of Establishment and Maintenance 265
III. Specific Cell Line Characteristics 268
References 278
Chapter 10. Cell Lines from Esophageal Tumors 282
I. Introduction 282
II. Methods of Establishment and Maintenance 283
III. Morphology 286
IV. Other Characteristics 290
V. Discussion 292
VI. Future Prospects 295
Acknowledgment 295
References 295
Chapter 11. Gastric Tumor Cell Lines 300
I. Introduction 300
II. Methods of Establishment and Maintenance 301
III. Morphology 305
IV. Other Characteristics 322
V. Future Prospectives 325
References 327
Chapter 12. Colorectal Cancer Cell Lines 330
I. Introduction 330
II. Establishment of Cell Lines 333
III. Culture Characteristics 337
IV. Morphological Characteristics 338
V. Other Characteristics 347
References 351
Chapter 13. Cell Lines from Urinary Bladder Tumors 354
I. Background 355
II. Patient History and History of Original Tumor 355
III. Histological Examination of the Original Tumors and Developed Cell Cultures 360
IV. Establishment and Maintenance of Bladder Cancer Lines 366
V. Mycoplasma Testing 366
VI. Cryopreservation of Cell Lines 366
VII. Characterization of Lines 367
VIII. Summary 372
Acknowledgments 373
References 373
Chapter 14. The Female Reproductive System: Cell Lines from Tumors of the Human Ovary and Uterus 376
I. Introduction 376
II. Methods of Establishment and Maintenance of Cell Lines 377
III. Morphological Aspects 380
IV. Growth and Other Characteristics 394
V. Future Prospects 401
Acknowledgments 401
References 401
Chapter 15. The Male Reproductive System: Prostatic Cell Lines 404
I. Introduction 404
II. Methods of Establishment and Maintenance 405
III. Morphology 408
IV. Other Characteristics 413
V. Discussion and Future Prospects 422
References 424
Chapter 16. Melanocyte and Melanoma Cell Lines 430
I. Introduction 430
II. Establishment and Maintenance 431
III. Morphology of Cultured Melanocytes and Melanoma Cells 433
IV. Growth Characteristics 433
V. Growth Factor Production by Melanoma Cells 436
VI. Chromosomal Abnormalities in Melanoma Cells 437
VII. Antigen Expression by Melanocytic Cells 438
VIII. Invasion and Metastasis of Melanoma Cells 439
IX. Conclusions 441
X. Origin of Cell Lines 442
Acknowledgement 443
References 443
Chapter 17. Exocrine Pancreatic Tumor Cell Lines 446
I. Introduction 446
II. Methods of Establishment and Maintenance 449
III. Morphology 449
IV. Other Characteristics 453
V. Induction of Differentiation 453
VI. Discussion 456
VII. Future Prospects 457
References 457
Chapter 18. Cell Lines from Human Germ-Cell Tumors 460
I. Introduction 460
II. Establishment and Maintenance of Cell Lines 462
III. Morphology 468
IV. Other Characteristics 475
V. Discussion and Future Prospects 480
VI. Reported Cell Lines Derived from Human Germ-Cell Tumors 483
Acknowledgments 486
References 487
Index 494