Advances in Protein Chemistry (eBook)

Front Cover 1
Advances in Protein Chemistry, Volume 47 4
Copyright Page 5
Contents 6
Contributors 10
Preface 12
Chapter 1. Hemocyanins 14
Prologue 14
I. Oxygen Transport Proteins: An Overview 15
II. Three-Dimensional Structures of Hemocyanin Molecules 18
III. The Oxygen-Binding Site 54
IV. Oxygen-Binding Equilibria 63
V. Synthesis of Hemocyanins 75
VI. Evolution of Hemocyanins 77
References 87
Chapter 2. Molten Globule and Protein Folding 96
I. Introduction 96
II. Equilibrium Molten Globule State 99
III. Phase Transitions 125
IV. Kinetics of Protein Folding 155
V. Mechanism of Protein Folding 184
VI. Physiological Role of Molten Globule 208
VII. Conclusion 229
References 230
Chapter 3. Enthalpic Contribution to Protein Stability: Insights from Atom-Based Calculations and Statistical Mechanics 244
I. Introduction 244
II. Models for Protein Stability 248
III. Enthalpy of Protein Folding 254
IV. van der Waals Interactions in Proteins and Model Systems 262
V. Electrostatic Interactions in Proteins: Hydrogen Bond Contribution 272
Vl. Enthalpy of Solvation of Polar Groups: Breakdown of Surface Area Proportionality Assumption 282
VII. Concluding Discussion 296
Appendix: Computational Details 301
References 306
Epilogue 309
Chapter 4. Energetics of Protein Structure 320
List of Symbols 321
I. Introduction 321
II. Structural Characteristics of Analyzed Proteins 326
III. Thermodynamic Characteristics of Analyzed Proteins 333
IV. Net Hydration Effects of Protein Groups 346
V. Hydration Effects on Protein Unfolding 357
VI. Entropy of Protein Unfolding in Vacuum 365
VII. Enthalpy of Protein Unfolding in Vacuum 378
VIII. lnteractions in Proteins 381
IX. Protein Unfolding in Aqueous Media 392
X. Contribution of Hydrogen Bonding in Stabilizing Protein Structure in Water 399
XI. Hydrophobic Interactions in Proteins 402
XII. Role of Packing of Groups in Protein Interior 408
XIII. Molten Globule State 412
XIV. Balance of Forces Stabilizing and Destabilizing Protein Structure 415
XV. Concluding Remarks 419
References 421
Epilogue 430
Author Index 440
Subject Index 461