Landmark Papers in Clinical Chemistry (eBook)

Front Cover 1
Landmark Papers in Clinical Chemistry 4
Copyright Page 5
Contents 6
Foreword 12
Introduction 14
Acknowledgments 16
Claude Bernard 18
Section I: Immunoassay Technology 20
RIA (Radioimmunoassay) 22
1.Immunoassay of endogenous plasma insulin in man. Commentary 22
Landmark Paper 24
FPIA (Fluorescence Polarization Immunoassay) 44
2. Quantification of the antigen-antibody reaction by the polarization of fluorescence. Commentary 44
Landmark Paper 46
CPB (Competitive Protein Binding) 52
3. Determination of thyroxine utilizing the property of protein-binding. Commentary 52
Landmark Paper 54
ELISA (Enzyme-Linked Immunosorbent Assay) 64
4. Enzyme-linked immunosorbent assay II. Commentary 64
Landmark Paper 54
EMIT™ (Enzyme Immunoassay Technique) 74
5. "Homogeneous" enzyme immunoassay. A new immunochemical technique. Commentary 74
Landmark Paper 76
Section II: Therapeutic Drug Monitoring (TDM) 82
Bromide 84
6. Rational bromide treatment. New methods for its control. Commentary 84
Landmark Paper 86
Sulfanilamide 92
7. A new coupling component for sulfanilamide determination. Commentary 92
Landmark Paper 94
Quinacrine 108
8. The estimation of Atabrine in biological fluids and tissues. Commentary 108
Landmark Paper 110
Digoxin 130
9. Determination of therapeutic and toxic serum digoxin concentrations by radioimmunoassay. Commentary 130
Landmark Paper 132
Theophylline 138
10. Determination of theophylline and its metabolites in human urine and serum by high-pressure liquid chromatography. Commentary 138
Landmark Paper 140
Section III: Enzymology 150
ALP (Alkaline Phosphatase, EC 3.1.3.1) 152
11. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. Commentary 152
Landmark Paper 154
AST (Aspartate Aminotransferase, EC 2.6.1.1) 164
12. Transaminase activity in human blood. Commentary 164
Landmark Paper 166
CK (Creatine Kinase, EC 2.7.3.2) 174
13. Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography. Commentary 174
Landmark Paper 176
Section IV: Specific Analytes 182
Colorimetric Analysis with the Duboscq 184
14. A system of blood analysis. Commentary 184
Landmark Paper 186
Blood Gases by Manometer 216
15. The determination of gases in blood and other solutions by vacuum extraction and manometric measurement. I. Commentary 216
Landmark Paper 218
Bilirubin With the Filter Photometer 238
16. The determination of bilirubin with the photoelectric colorimeter. Commentary 238
Landmark Paper 240
Proteins by the Biuret Reaction 250
17. Determination of serum proteins by means of the biuret reaction. Commentary 250
Landmark Paper 252
Methods for Pediatric and Neonatal Samples 268
18. Routine use of ultramicro methods in the clinical laboratory. Commentary 268
Landmark Paper 270
Enzymatic Glucose 290
19. Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose. Commentary 290
Landmark Paper 292
HCG (Human Chorionic Gonadotropin) by Immunoassay 296
20. An immunological pregnancy test. Commentary 296
Landmark Paper 298
Hemoglobin Alc 306
21. An abnormal hemoglobin in red cells of diabetics. Commentary 306
Landmark Paper 308
LDL (Low Density Lipoprotein) Cholesterol by Calculation 312
22. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Commentary 312
Landmark Paper 314
Section V: Instrumentation and Techniques 318
Paper Electrophoresis 320
23. A microelectrophoretic and microionophoretic technique. Commentary 320
Landmark Paper 322
Automated Analyzer 328
24. An automated method for colorimetric analysis. Commentary 328
Landmark Paper 330
Enzymatic Glucose, Dry Reagents on Pads 342
25. Simple specific test for urine glucose. Commentary 342
Landmark Paper 346
Blood Gas Electrodes 352
26. Electrodes for blood pO2 and pCO2 determination. Commentary 352
Landmark Paper 354
Specific Ion Electrodes 360
27. Single sample analysis with the sodium electrode. Commentary 360
Landmark Paper 362
Centrifugal Analyzer 370
28. Analytical techniques for cell fractions XII. A multiple-cuvet rotor for a new microanalytical system. Commentary 370
Landmark Paper 372
Immunoassays on Membranes 390
29. Immunocapillarymigration—A new method for immunochemical quantitation. Commentary 390
Landmark Paper 392
Western Blots 400
30. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Commentary 400
Landmark Paper 404
Capillary Electrophoresis (CE) 410
31. Zone electrophoresis in open-tubular glass capillaries. Commentary 410
Landmark Paper 412
Section VI: Chemometrics 418
Quality Control Charts 420
32. The use of control charts in the clinical laboratory. Commentary 420
Landmark Paper 422
Interferences in Analytical Accuracy 430
33. Chemical and diagnostic specificity of laboratory tests. Commentary 430
Landmark Paper 432
Predictive Value Theory 452
34. Predictive value of a single diagnostic test in unselected populations. Commentary 452
Landmark Paper 454
Standards and Calibrators 458
35. What is a standard? Commentary 458
Landmark Paper 460
Test Kits 482
36. Performance of "kits" used for clinical chemical analysis of cholesterol. Commentary 482
Landmark Paper 484
Section VII: Molecular Diagnostics 490
Biochemical Genetics 492
37. Sickle cell anemia, a molecular disease. Commentary 492
Landmark Paper 494
Nucleic Acid Probes 500
38. Detection of Chlamydia trachomatis in clinical specimens by nucleic acid spot hybridization. Commentary 500
Landmark Paper 502
Microarrays 506
39. Oligonucleotide hybridisations on glass supports: a novel linker for oligonucleotide synthesis and hybridisation properties of aoligonucleotides synthesised in situ. Commentary 506
Landmark Paper 508
Overview of the Publication Source of the Original Articles 514
Author Index 518
Subject Index 520