Landmark Papers in Clinical Chemistry

Following a 25-year career in clinical chemistry, R M Rocco currently teaches graduate courses in the biomedical laboratory sciences at San Francisco State University. Landmark Papers grew out of a graduate seminar in landmark papers in biotechnology. Dr Rocco has extensive experience in directing pharmaceutical and clinical chemistry research and development groups.

Preface
Section IIMMUNOASSAY TECHNOLOGY
RIA (Radioimmunoassay)
1. Yalow, R.S., Berson, S.A. (1960: Assay of plasma insulin in man.
FPIA (Fluorescence Polarization Immunoassay)
2. Dandliker, W.B., Feigen, G.A. (1961): Quantification of the antigen-antibody reaction by the polarization of fluorescence.
CPB (Competitive Protein Binding)
3. Murphy, B.E.P., Pattee, C.J. (1964): Determination of thyroxine utilizing the property of protein-binding.
ELISA (Enzyme-Linked Immunosorbent Assay)
4. Engvall, E., Perlmann, P. (1972): Enzyme-linked immunosorbent assay, ELISA. III.
EMITä (Enzyme Immunoassay Technique)
5. Rubenstein, K.E., Schneider, R. S., Ullman, E.F. (1972): "Homogeneous" enzyme immunoassay.

Section IITHERAPEUTIC DRUG MONITORING (TDM)
Bromide
6. Wuth, O. (1927): Rational bromide treatment.
Sulfonamides
7. Bratton, A.C., Marshall, E.K., Jr., (1939): A New Coupling Component for Sulfanilamide Determination.
Quinine and Quinidine
8. Brodie, B.B., Udenfriend, S. (1943): The estimation of quinine in human plasma with a note on the estimation of quinidine.
Digoxin
9. Smith, T.W., Butler, V.P., Haber, E. (1969): Determination of therapeutic and toxic serum digoxin concentrations by radioimmunoassay.
Theophylline
10. Thompson, R.D., Nagasawa, H.T., Jenne, J.W. (1974): Determination of theophylline and its metabolites in human urine and serum by high-pressure liquid chromatography.

Section IIIENZYMOLOGY
ALP (Alkaline Phosphatase, EC 3.1.3.1))
11. Bessey, O.A., Lowry, O.H., Brock, M.J. (1946): A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum.
AST (Aspartate Aminotransferase, EC 2.6.1.1)
12. Karmen, A., Wroblewski, F., LaDue, J.S. (1955): Transaminase activity in human blood.
CK (Creatine Kinase, EC 2.7.3.2)
13. Mercer, D.W. (1974): Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography.

Section IV SPECIFIC ANALYTES
Colorimetric Analysis with the Duboscq
14. Folin, O., Wu, H. (1919): A system of blood analysis.
Blood Gases by Manometer
15. Van Slyke, D.D. (1924): The determination of gases in blood and other solutions by vacuum extraction and manometric measurement. I.
Bilirubin, Total and Direct With the Filter Photometer
16. Malloy, H.T., Evelyn, K.A. (1937): The determination of bilirubin with the photometric colorimeter.
Proteins by the Biuret Reaction
17. Gornall, A. G., Bardawill, C. J., David, M. M. (1949): Determination of serum proteins by means of the Biuret reaction.
Methods for Pediatric and Neonatal Samples
18. Natelson, S. (1951): Routine use of ultramicro methods in the clinical laboratory.
Enzymatic Glucose
19. Huggett, A.S., Nixon, D.A. (1957): Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose.
HCG (Human Chorionic Gonadotropin) by Immunoassay
20. Wide, L., Gemzell, C.A. (1960): An immunological pregnancy test.
Hemoglobin A1c
21. Rahbar, S. (1968): An Abnormal Hemoglobin in Red Cells of Diabetics.
LDL (Low Density Lipoprotein) Cholesterol by Calculation
22. Friedewald, W.T., Levy, R.I., Fredrickson, D.S. (1972): Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge.

Section V INSTRUMENTATION AND TECHNIQUES
Paper Electrophoresis
23. Durrum, E.L. (1950): A microelectrophoretic and microionophoretic technique.
Automated Analyzer
24. Skeggs, L.T. (1957): An automatic method for colorimetric analysis.
Enzymatic Glucose, Dry Reagents on Pads
25. Free, A.H., Adams, E.C., Kercher, M.L., Free, H. M., Cook, M. H., (1957): Simple Specific Test for Urine Glucose.
Blood Gas Elelctrodes
26. Severinghaus, J., Bradley, A.F. (1958): Electrodes for blood pO2 and pCO2 determination.
Specific Ion Electrodes
27. Friedman, S.M., Nakashima, M. (1961): Single sample analysis with the sodium electrode.
Centrifugal Analyzer
28. Anderson, N.G. (1969): Analytical techniques for cell fractions. XII. A multiple-cuvet rotor for a new microanalytical system.
Immunoassays on Membranes
29. Glad, C., Grubb, A.O. (1978): Immunocapillary migration- A new method for immunochemical quantitation.
Western Blots
30. Towbin, H., Stachelin, T., Gordon, J. (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications.
Capillary Electrophoresis (CE)
31. Jorgenson, J.W., Lukacs, K. DeA. (1981): Zone electrophoresis in open-tubular glass capillaries.

Section VI CHEMOMETRICS
Quality Control Charts
32. Levey, S., Jennings, E.R. (1950): The Use of Control Charts in the Clinical Laboratory.
Interferences in Analytical Accuracy
33. Caraway, W. (1962): Chemical and Diagnostic Specificity of Laboratory Tests.
Predictive Value Theory
34. Vecchio, T.J. (1966): Predictive value of a single diagnostic test in unselected populations.
Standards and Calibrators
35. Radin, N. (1967): What is a standard?
Test Kits
36. Barnett, R.N., Cash, A.D., Junghams, S.P. (1968): Performance of "kits" used for clinical chemical analysis of cholesterol.

Section VII MOLECULAR DIAGNOSTICS
Biochemical Genetics
37. Pauling, L., Itano, H.A., Singer, S.J., Wells., I.C. (1949): Sickle cell anemia, a molecular disease.
Nucleic Acid Probes
38. Hyypia, T., Jalava, A., Larsen, S.H., Terho, P., Hukkanene, V. (1985): Detection of Chlamydia trachomatis in clinical specimens by nucleic acid spot hybridization.
Microarrays
39. Maskos, U., Southern, E.M. (1992): Oligonucleotide hybridizations on glass supports: A novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotide synthesized in situ.

Acknowledgments
Author Index
Subject Index