Plant-Virus Interactions

Computational Pipeline for Detection of Plant RNA Viruses Using High-Throughput Sequencing.- Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons.- Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral cDNAs (icDNAs).- Construction of Geminivirus Infectious Clones for Agroinoculation into Plants.- Agroinoculation of Geminiviral Infectious Clones into Plants.- Begomovirus Inoculation in Arabidopsis and Cassava.- Absolute and Relative Quantification of Single-Stranded Positive Sense RNA Virus from Plant Tissue.- Quantification of Virion-Sense and Complementary-Sense DNA Strands of Circular Single-Stranded DNA Viruses.- Replication Assay of Begomovirus in Arabidopsis Protoplasts.- Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye.- Ribosome Profiling of Plants.- Experimental Transmission of Plant Viruses by Aphids or Whiteflies.- Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana.- Protein-Protein Interaction via Two-Hybrid Assay in Yeast.- Bimolecular Fluorescence Complementation (BIFC) in Host-Virus Interactions.- Co-Immunoprecipitation Assays to Detect Protein-Protein Interactions.- Split-Luciferase Complementation Imaging Assay in Virus-Plant Interactions.- The Split-Luciferase Complementation Assay to Detect and Quantify Protein-Protein Interactions In Planta.- TurboID-Based Proximity Labeling: A Method to Decipher Protein-Protein Interactions in Plants.- Virus-Induced Heritable Gene Editing in Plants.- Immunoprecipitation Followed by Mass Spectrometry: An Approach for Identifying Host-Viral Protein-Protein Interactions.- In Situ Protein Microarray for Identifying the Geminivirus-Arabidopsis Interactome.