Plant-Virus Interactions

lt;p>1. Computational Pipeline for Detection of Plant RNA Viruses Using High-Throughput Sequencing

            Livia Donaire and Miguel A. Aranda

2. Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons

            João Paulo Herrera da Silva and F. Murilo Zerbini

3. Initiation of Plant Virus Infections by Agroinfiltration of Infectious Viral cDNAs (icDNAs)

            Maija Pollari

4. Construction of Geminivirus Infectious Clones for Agroinoculation into Plants

            Ivair J. Morais, Alice K. Inoue-Nagata, and Erich Y.T. Nakasu

5. Agroinoculation of Geminiviral Infectious Clones into Plants

            Ivair J. Morais, Yanca F. M. Ferreira, Erich Y.T. Nakasu, Tatsuya Nagata, and Alice K. Inoue-Nagata

6. Begomovirus Inoculation in Arabidopsis and Cassava

            José T. Ascencio-Ibáñez, Mary M. Dallas, and Linda Hanley-Bowdoin

7. Absolute and Relative Quantification of Single-Stranded Positive Sense RNA Virus from Plant Tissue

            Pinky Dutta and Kristiina Mäkinen

8. Quantification of Virion-Sense and Complementary-Sense DNA Strands of Circular Single-Stranded DNA Viruses

            Edgar A. Rodríguez-Negrete and Ana Grande-Pérez

9. Replication Assay of Begomovirus in Arabidopsis Protoplasts

            Laura Gonçalves Costa Martins and Elizabeth P.B. Fontes

10. Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye

            Yinzi Li and Aiming Wang

11. Ribosome Profiling of Plants

            Jahanara Sonia, Pulkit Kanodia, Zachary Lozier, and W. Allen Miller

12. Experimental Transmission of Plant Viruses by Aphids or Whiteflies

            Irene Ontiveros, Juan Antonio Diaz-Pendón, and Juan José López-Moya

13. Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana

            Miriam Gil-Valle, Cristina Sáez, Nuria Montes, and Israel Pagán

14. Protein-Protein Interaction via Two-Hybrid Assay in Yeast

            Christiane Eliza Motta Duarte and Nivea Costa Euclydes

15. Bimolecular Fluorescence Complementation (BIFC) in Host-Virus Interactions

            Fredy Davi Albuquerque Silva, João Paulo Batista Machado, and Pedro Augusto Braga dos Reis

16. Co-Immunoprecipitation Assays to Detect Protein-Protein Interactions

            Mengling Huang, Xiao Yu, and Bo Li

17. Split-Luciferase Complementation Imaging Assay in Virus-Plant Interactions

            Huang Tan, Xi Zhang, and Rosa Lozano-Duran

18. The Split-Luciferase Complementation Assay to Detect and Quantify Protein-Protein Interactions In Planta

            Eduardo Bassi Simoni and Célio Cabral Oliveira

19. TurboID-Based Proximity Labeling: A Method to Decipher Protein-Protein Interactions in Plants

            Yuanyuan Li, Yongliang Zhang, and Savithramma P. Dinesh-Kumar

20. Virus-Induced Heritable Gene Editing in Plants

            Ugrappa Nagalakshmi, Nathan Meier, and Savithramma P. Dinesh-Kumar

21. Immunoprecipitation Followed by Mass Spectrometry: An Approach for Identifying Host-Viral Protein-Protein Interactions

            Laura Medina-Puche and Rosa Lozano-Duran

22. In Situ Protein Microarray for Identifying the Geminivirus-Arabidopsis Interactome

            Junshi Yazaki and Maximiller Dal-Bianco