YAC Protocols

Markers, Selection, and Media in Yeast Artificial Chromosome Cloning.- Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms.- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization.- Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction.- Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence.- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes.- Modification and Amplification of Yeast Artificial Chromosomes.- Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions.- Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes.- A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones.- Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes.- Targeting Vector Construction by Yeast Artificial Chromosome Modification.- Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos.- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection.- Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals.- Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization.- Contribution of Yeast Artificial Chromosome-Based Physical Maps to the FinalAssembly of the Trypanosoma cruzi Genome.