YAC Protocols
Humana Press Inc. (Verlag)
978-1-61737-673-3 (ISBN)
Markers, Selection, and Media in Yeast Artificial Chromosome Cloning.- Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms.- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization.- Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction.- Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence.- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes.- Modification and Amplification of Yeast Artificial Chromosomes.- Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions.- Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes.- A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones.- Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes.- Targeting Vector Construction by Yeast Artificial Chromosome Modification.- Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos.- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection.- Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals.- Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization.- Contribution of Yeast Artificial Chromosome-Based Physical Maps to the FinalAssembly of the Trypanosoma cruzi Genome.
Erscheint lt. Verlag | 10.12.2010 |
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Reihe/Serie | Methods in Molecular Biology ; 349 |
Zusatzinfo | 49 Illustrations, black and white; XVI, 204 p. 49 illus. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 152 x 229 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Biochemie | |
ISBN-10 | 1-61737-673-6 / 1617376736 |
ISBN-13 | 978-1-61737-673-3 / 9781617376733 |
Zustand | Neuware |
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