The Mitotic Spindle
Springer-Verlag New York Inc.
978-1-0716-4223-8 (ISBN)
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Authoritative and up-to-date, The Mitotic Spindle: Methods and Protocols serves as an ideal guide for researchers seeking a better system-level understanding of the mitotic spindle.
Chapters 4, 12, 17, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy.- Chromosome Counting at Meiosis and Mitosis of Mouse Oocytes and Embryos Using Super-Resolution Live-Cell Imaging and CRISPR/dCas9-Mediated Live-FISH.- Cell Division Imaging of Tobacco BY-2 Cells in a 3D Volume by Two-Photon Spinning-Disk Confocal Microscopy.- Spatial Statistics of Three-Dimensional Growth Dynamics of Spindle Microtubules.- A Centrifuge Polarizing Microscope (CPM) that Enables the Visualization of Intracellular Structures Under High Centrifugal Forces.- Manipulation of Spindle Position Using Magnetic Tweezers in Sea Urchin Embryos.- Photochemical Control of Cell Division Using a Photoswitchable CENP-E Inhibitor.- Quantitatively Analyzing the Morphological Growth Dynamics of Bipolar and Non-Bipolar Spindles Using Xenopus Egg Cytoplasmic Extract.- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.- 4D Microscopy and Tracking of Chromosomes and the Spindle in C. elegans Early Embryos.- Assessment of Chromosome Oscillations in Mammalian Cells by Live Cell Imaging.- Genetic Engineering and Screening Using Base Editing and Inducible Gene Knockout.- Systematic Identification of Microtubule Post-Translational Modification “Readers” by Quantitative Proteomics.- Comparative Pharmacological Analysis of Mitotic Inhibitors Using Isogenic Ploidy Series of HAP1 Cells.- A Method for Analyzing Acentrosomal Mitotic Spindles in Human Cells.- Isolation of Mitotic Centrosomes from Cultured Human Cells.- Synthetic Engineering of Cortical Polarity During Mitosis Using Designed Proteins.- High-Resolution Imaging of Spindle Orientation Dynamics in 3D Intestinal Organoids.- Time-Lapse Imaging of Asymmetric Spindle Positioning During Endothelial Tip Cell Division in Angiogenesis In Vivo.
Erscheint lt. Verlag | 31.1.2025 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 76 Illustrations, color; 6 Illustrations, black and white; Approx. 275 p. 81 illus., 76 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-0716-4223-5 / 1071642235 |
ISBN-13 | 978-1-0716-4223-8 / 9781071642238 |
Zustand | Neuware |
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