Methods for Plant Nucleus and Chromatin Studies

Whole-Genome Bisulfite Sequencing with a Small Amount of DNA.- Quantitative profiling of Histone variants and post-translational modifications by Tandem Mass Spectrometry in Arabidopsis.- Hi-ChIP for plant tissues.- A practical approach to high-throughput and accurate mapping-by-sequencing in Arabidopsis.- ChIP-Rx: Arabidopsis chromatin profiling using quantitative ChIP-seq.- Chromatin Immunoprecipitation for standard, rare or weakly-binding proteins.- Isolation and identification of Nuclear Protein Complexes Using GFP-tagged Arabidopsis Lines and IP-MS Approach.- Identification of Plant Chromatin Interaction Networks Using IP-MS and co-IP.- Unraveling Plant Nuclear Envelope Composition Using Proximity Labeling Proteomics.- A method for characterizing protein-histone peptide interactions in vitro.- Detection and automated quantification of nucleocytoplasmic RNA fractions in Arabidopsis using smFISH.- Simultaneous visualization of protein and genomic regions in plant nuclei.- Microfluidics to follow spatio-temporal dynamics at the nucleo-cytoplasmic interface during plant root growth.- Characterizations of nuclear organization and chromatin modifications in mature embryos from Arabidopsis dry seeds.- 3D STED Imaging of Isolated Arabidopsis thaliana Nuclei.- Understanding and simulating the dynamics of a polymer like chromatin.- Technical Mini-Review: dCas-based tools to visualize chromatin or modify epigenetic marks at specific plant genomic loci.- Using Callus as an Ex-Vivo System for Chromatin Analysis.