Plant Protein Secretion
Springer-Verlag New York Inc.
978-1-0716-4058-6 (ISBN)
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Cutting-edge and comprehensive, Plant Protein Secretion: Methods and Protocols, Second Edition is a valuable resource for researchers and students in the field of plant biology, and will inspire further advancements in our understanding of protein secretion in plant cells and beyond.
Conventional and Unconventional Protein Secretion in Yeast and Animal Cells.- An Overview of Protein Secretion in Plant Cells.- Bioinformatic Analysis for Identifying Transcription Factors Involved in Protein Secretion and Vacuole Formation.- Using AlphaFold2 and Molecular Dynamics Simulation to Model Protein Recognition.- Proteomic Analysis of Secreted Vesicle Proteins from Plant Cells.- Extracellular Vesicle Isolation and Mass-Spectrometry-Based Proteomic Analysis in Arabidopsis thaliana.- Polysaccharides Analysis of Cell Wall Using Carbohydrate Gel Electrophoresis.- Acetyl Bromide Method for Total Lignin Content Determination in Plant Biomass.- Utilizing Plant Protein Secretion System for Pharmaceutical Protein Production.- In Vitro Reconstitution of Plant COPII Vesicles.- Identification of Neighboring Proteins of Endosomal Regulators by Using TurboID-Based Proximity Labeling.- Quantification of Protein Dynamics in Growing Pollen Tubes via Whole Cell Kymograph Analysis.- Polar Localization Analysis of Anionic Phospholipids and Their Binding Proteins in Arabidopsis Pollen Tubes.- Vacuum-Based Agroinfiltration for Transient Gene Expression in Arabidopsis Seedlings.- Pharmaceutical Analysis in the Plant Endomembrane System.- Observation of Vacuoles in Arabidopsis Developing Pollen Grain with Whole-Cell Electron Tomography.- Whole-Cell Electron Tomography Analysis of Vacuole Biogenesis.- Microscopy Analysis of Autophagosomal Structures in Plant Cells.- Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.- High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.- Determination of the Cryo-EM Structure of ATG9 from Arabidopsis thaliana.- Genetic Screening of Factors in the Plant Protein Secretion.- Studying Autophagy and Senescence of Arabidopsis Stigmatic Cells.
Erscheinungsdatum | 09.08.2024 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 49 Illustrations, color; 7 Illustrations, black and white; XII, 250 p. 56 illus., 49 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Schlagworte | Bioinformatics • Electron Tomograms • homology modeling • Plant Golgi Stacks • Vacuole Formation |
ISBN-10 | 1-0716-4058-5 / 1071640585 |
ISBN-13 | 978-1-0716-4058-6 / 9781071640586 |
Zustand | Neuware |
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