Plant Protein Secretion

Conventional and Unconventional Protein Secretion in Yeast and Animal Cells.- An Overview of Protein Secretion in Plant Cells.- Bioinformatic Analysis for Identifying Transcription Factors Involved in Protein Secretion and Vacuole Formation.- Using AlphaFold2 and Molecular Dynamics Simulation to Model Protein Recognition.- Proteomic Analysis of Secreted Vesicle Proteins from Plant Cells.- Extracellular Vesicle Isolation and Mass-Spectrometry-Based Proteomic Analysis in Arabidopsis thaliana.- Polysaccharides Analysis of Cell Wall Using Carbohydrate Gel Electrophoresis.- Acetyl Bromide Method for Total Lignin Content Determination in Plant Biomass.- Utilizing Plant Protein Secretion System for Pharmaceutical Protein Production.- In Vitro Reconstitution of Plant COPII Vesicles.- Identification of Neighboring Proteins of Endosomal Regulators by Using TurboID-Based Proximity Labeling.- Quantification of Protein Dynamics in Growing Pollen Tubes via Whole Cell Kymograph Analysis.- Polar Localization Analysis of Anionic Phospholipids and Their Binding Proteins in Arabidopsis Pollen Tubes.- Vacuum-Based Agroinfiltration for Transient Gene Expression in Arabidopsis Seedlings.- Pharmaceutical Analysis in the Plant Endomembrane System.- Observation of Vacuoles in Arabidopsis Developing Pollen Grain with Whole-Cell Electron Tomography.- Whole-Cell Electron Tomography Analysis of Vacuole Biogenesis.- Microscopy Analysis of Autophagosomal Structures in Plant Cells.- Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.- High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.- Determination of the Cryo-EM Structure of ATG9 from Arabidopsis thaliana.- Genetic Screening of Factors in the Plant Protein Secretion.- Studying Autophagy and Senescence of Arabidopsis Stigmatic Cells.