Epizootic Hemorrhagic Disease Virus

Managing risk when working with Orbiviruses with a special emphasis on Epizootic haemorrhagic disease virus.- Recognition of Field Signs, Necropsy Procedures and Evaluation of Macroscopic Lesions of Cervids Infected with Epizootic Hemorrhagic Disease Virus.- Virus Isolation of Epizootic Haemorrhagic Disease Virus in Cell Culture.- Purification of epizootic haemorrhagic disease virus (and other Orbiviruses) particles from infected mammalian or insect cells.- Confocal microscopy of fluorescently labelled EHDV infected cell cultures.- Epizootic Haemorrhagic Disease Virus Titration.- Virus neutralisation test for detecting and quantifying serum neutralising antibodies to epizootic haemorrhagic disease virus (serotypes 1, 2, and 4-8).- Epizootic Hemorrhagic Disease Agar Gel Immunodiffusion Assay.- Detection of EHDV antibodies using enzyme-linked immunosorbent assay (ELISA).- Real-time RT-PCR for the diagnosis of epizootic haemorrhagic disease virus.- Real-time RT-PCR assays for typing of Epizootic Haemorrhagic Disease virus.- Rapid Laboratory Diagnosis of Epizootic Haemorrhagic Disease Virus using a Loop Mediated Isothermal Amplification Assay.- End-point RT-PCR assays for detection and typing of Epizootic Haemorrhagic Disease virus.- Molecular serotyping of epizootic haemorrhagic disease virus.-Whole genome sequencing of Epizootic Haemorrhagic Disease Virus.- Infection of Culicoides biting midges with Epizootic Haemorrhagic Disease Virus.