Oxygen Sensing

Integrating UV-Vis Spectroscopy and Oxygen Optode for Accurate Determination of Oxygen Affinity of Proteins.- Measurement of O2 Binding by Sensory Hemeproteins.- Resonance Raman Characterization of O2-Binding Heme Proteins.- Anaerobic Infra-Red Spectroelectrochemical Methods for Studying Oxygen-Sensitive [FeFe] Hydrogenases.- Monitoring the Kinase Activity of Heme-Based Oxygen Sensors and Its Dependence on O2 and Other Ligands using Phos-tag Electrophoresis.- In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity.- Spectrophotometric Method for the Quantification and Kinetic Evaluation of In Vitro c-di-GMP Hydrolysis in the Presence and Absence of Oxygen.- Hydrogen/Deuterium Exchange Mass Spectrometry of Heme-Based Oxygen Sensor Proteins.- Methods for Biophysical Characterization of SznF, A Member of the Heme-Oxygenase-Like Diiron Oxidase/Oxygenase Superfamily.- Analyzing Iron and Oxygen-Regulated ProteinComplex Formation using Proteomic Mass Spectrometry.- CRISPR Activator Approaches to Study Endogenous Androglobin Gene Regulation.- Assays to Study Hypoxia-Inducible Factor Prolyl Hydroxylase Domain 2 (PHD2), A Key Human Oxygen Sensing Protein.- Kinetic Measurement Investigating the Oxygen-Sensing Properties of the Plant Cysteine Oxidases.- Methods for Culturing Anaerobic Microorganisms.