Oxygen Sensing
Springer-Verlag New York Inc.
978-1-0716-3079-2 (ISBN)
Authoritative and comprehensive, Oxygen Sensing: Methods and Protocols is a valuable resource to a wide audience, ranging from microbiologists and cell biologists, to protein biochemists.
Integrating UV-Vis Spectroscopy and Oxygen Optode for Accurate Determination of Oxygen Affinity of Proteins.- Measurement of O2 Binding by Sensory Hemeproteins.- Resonance Raman Characterization of O2-Binding Heme Proteins.- Anaerobic Infra-Red Spectroelectrochemical Methods for Studying Oxygen-Sensitive [FeFe] Hydrogenases.- Monitoring the Kinase Activity of Heme-Based Oxygen Sensors and Its Dependence on O2 and Other Ligands using Phos-tag Electrophoresis.- In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity.- Spectrophotometric Method for the Quantification and Kinetic Evaluation of In Vitro c-di-GMP Hydrolysis in the Presence and Absence of Oxygen.- Hydrogen/Deuterium Exchange Mass Spectrometry of Heme-Based Oxygen Sensor Proteins.- Methods for Biophysical Characterization of SznF, A Member of the Heme-Oxygenase-Like Diiron Oxidase/Oxygenase Superfamily.- Analyzing Iron and Oxygen-Regulated ProteinComplex Formation using Proteomic Mass Spectrometry.- CRISPR Activator Approaches to Study Endogenous Androglobin Gene Regulation.- Assays to Study Hypoxia-Inducible Factor Prolyl Hydroxylase Domain 2 (PHD2), A Key Human Oxygen Sensing Protein.- Kinetic Measurement Investigating the Oxygen-Sensing Properties of the Plant Cysteine Oxidases.- Methods for Culturing Anaerobic Microorganisms.
Erscheinungsdatum | 14.04.2023 |
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Reihe/Serie | Methods in Molecular Biology ; 2648 |
Zusatzinfo | 47 Illustrations, color; 8 Illustrations, black and white; X, 240 p. 55 illus., 47 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | affinity measurements • Biofilm • cyclase kinetics • FixL-FixJ • heme sensor proteins |
ISBN-10 | 1-0716-3079-2 / 1071630792 |
ISBN-13 | 978-1-0716-3079-2 / 9781071630792 |
Zustand | Neuware |
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