Rift Valley Fever Virus

An Introduction to Rift Valley Fever Virus.- Aedes aegypti Vector Competence Assay for Rift Valley Fever Virus using Artificial Blood Meal.- Isolation of Rift Valley Fever Virus from Field Specimens using Cell Culture Approaches.- Detection and Diagnosis of Rift Valley Fever Virus.- Analysis of Negative-Strand RNA Viruses by RT-qPCR: Rift Valley Fever Virus and Toscana Virus.- Validated Methods for Effective Decontamination and Inactivation of Rift Valley Fever Virus.- Guidelines for In Vitro Production and Quantification of Rift Valley Fever Virus.- Cell-Free Synthesis of Bunyavirales Proteins in View of Their Structural Characterization by Nuclear Magnetic Resonance.- Expression and Purification of RVFV Glycoprotein C for Structural Studies using Baculovirus Expression System.- Next-Generation Sequencing and Proteomics-Enabled Approach for Rapid and High-Throughput Isolation of Virus-Neutralizing Nanobodies.- Use of Single-Domain Antibodies Against the NSm Protein for the Detection of Cells Infected by Rift Valley Fever Virus.- Making Rift Valley Fever Viral Particles Fluorescent.- Identification of RVFV Host Factors using Quantitative Interaction Proteomics.- Genome-Wide CRISPR-Cas9 Screening for the Identification of Host Dependency Factors of Emerging Viruses.- Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography.- Imaging Bunyavirus Infections by Transmission Electron Microscopy: Conventional Sample Preparation vs High-Pressure Freezing and Freeze-Substitution.- The Phlebovirus Ribonucleoprotein: An Overview.- Nucleotide Resolution Mapping of Rift Valley Fever Virus Nucleoprotein - Genome RNA Interactions.- Characterization of RVFV Nucleocapsid Protein Binding Sites on RNA by iCLIP-seq.- Yeast Expression and Activity Assay of RVFV Polymerase.- Single-Molecule Visualization of Bunyavirus Genome Segments using Fluorescence In Situ Hybridization.- Single-Cell RNA-Sequencing of RVFV Infection.- Using RNAseq to Uncover Transcriptional and Splicing Differences in Host Cells during Rift Valley Fever Virus Infection.- Quantification of RVFV Specific T Cell Responses in Mice Pre-Immunized with Potential Vaccine Candidates.- Use of Human Macrophages to Study Bunyavirus NSs Functions.- 3D Spheroid and Organoid Models to Study Neuroinfection of RNA Viruses.- Laboratory Animal Models for Rift Valley Fever Virus Disease.- Monitoring RVFV Infection using Bioluminescent Reporter Viruses In Vivo.