Connexin Hemichannels

Purification, Reconstitution, and Functional Analysis of Connexin Hemichannels.- Expression and Function of Connexins in Extracellular Vesicles.- Expression, Purification, and Nanodisc Reconstitution of Connexin-43 Hemichannels for Structural Characterization by Cryo-Electron Microscopy.- Molecular Dynamics Simulation of Permeation through Connexin Channels.- Spatial and Temporal Localization of Connexins in Cells Using Confocal Microscopy.- Assessment of Connexin43 Hemichannel Functionality Based on Cytosolic Uptake of Yo-Pro1.- A Dye Uptake Assay to Measure Large-Pore Channel Activity in Trypanosoma cruzi.- Measurement of Ca2+ Uptake through Connexin Hemichannels.- Evaluation of Connexin Hemichannel Activity In Vivo.- Protocol for the Study of Connexin and DNA Interactions.- Protocol for the Electrophysiological Characterization of Endogenous Pannexin 1 Intercellular Cell-Cell Channels.- Generation of Connexin-Expressing Stable Cell Pools.- A Protocol for the Automated Assessment of Cutaneous Pathology in a Mouse Model of Hemichannel Dysfunction.- Measuring Connexin Hemichannel Opening in Response to a InsP3-Mediated Cytosolic Ca2+ Increase.