Photorespiration

High throughput phosphoglycolate phosphatase activity assay using crude leaf extract and recombinant enzyme to determine kinetic parameters Km and Vmax using a microplate reader-  Measuring phosphoglycolate phosphatase activity using NMR detection of glycolate.- Determination of phosphoglycolate phosphatase activity via a coupled reaction using recombinant glycolate oxidase.- Spectrophotometric Assays for Measuring Photorespiratory Glutamate:Glyoxylate and Serine:Glyoxylate Aminotransferase Reactions.- Mitochondrial dihydrolipoamide dehydrogenase (mtLPD1) – expression, purification, activity and redox regulation.-Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity.- High throughput glycerate kinase activity assay using crude leaf extract and recombinant enzyme to determine kinetic parameters Km and Vmax using a microplate reader.- Purification of recombinant N-terminal histidine-tagged Arabidopsis thalianaphosphoglycolate phosphatase 1, glycolate oxidase 1 and 2, and hydroxypyruvate reductase 1.- Measuring and Quantifying Characteristics of the Post-Illumination Burst.- Low-cost system for maintaining low atmospheric CO2 levels during Arabidopsis cultivation.- Measuring the release of ammonia from leaves.- Using gas exchange to study CO2 release during photosynthesis with steady- and non-steady-state approaches.- Measurement of photorespiratory oxygen exchange by membrane inlet mass spectrometry.- Using Dynamic Gas Exchange Measurements During Oxygen Transients to Study Non-steady-state Photorespiration.- Analysis of photorespiratory intermediates under transient conditions by mass spectrometry.- Combining gas exchange and rapid quenching of leaf tissue for mass spectrometry and NMR analysis using an external chamber.- Combining gas exchange and rapid quenching of leaf tissue for mass spectrometry analysis directly in gas exchange cuvette.- Reaction-kinetic modeling of photorespiration using modelbase.- Using publicly available RNA-seq data for expression analysis of genes of interest.- Identification and functional characterization of cis-regulatory elements of key photorespiratory genes in response to short-term abiotic stress conditions.- Identification of auxiliary organellar targeting signals for plant peroxisomes using bioinformatic analysis of large protein sequence datasets followed by experimental validation.