Plant Functional Genomics
Springer-Verlag New York Inc.
978-1-0716-3781-4 (ISBN)
Authoritative and comprehensive, Plant Functional Genomics: Methods and Protocols serves as an ideal guide to the immense promise of functional genomics, spanning from fundamental evolutionary studies to practical advancements in crop improvement.
Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Carotenoid Extraction from Plant Tissues.- Sample Preparation from Plant Tissue for Gas Chromatography–Mass Spectrometry (GC–MS).- Identification of Volatiles in Tomato Fruit Using Headspace Solid-Phase-Micro-Extraction (HS-SPME) Coupled with Gas Chromatography-Mass Spectrometry (GC-MS).- Determination of Molecular Dimensions of Carbohydrate Polymers (Polysaccharides) by Size Exclusion Chromatography (SEC).- Monosaccharide Analysis after One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection.- Atomic Force Microscopy in the Characterization of the Structure of Cell Wall Components.- Studying Plant Specialized Metabolites Using Computational Metabolomics Strategies.- pyPGCF: A Python Software for Phylogenomic Analysis, Species Demarcation, Identification of Core and Fingerprint Proteins of Bacterial Genomes that Are Important for Plants.- Application of Integrated Computational Approaches in Prediction of Plant Virus Encoded miRNAs and Their Targeted Plant Genes.- Gene Co-Expression Network Analysis to Mine Genes Involved in Plant Secondary Metabolite Biosynthesis and Regulation.- Organic Supplementation of Vaccinium corymbosum Micropropagation Media.- Coffee Cell Suspensions as a Platform for Transient Gene Expression Analysis.- Agroinfiltration for Enhanced Transgene Expression in Coffee Leaves (Coffea arabica L.).- In Vitro Gamma Mutagenesis Techniques in Rice (Oryza sativa L. var. Lazarroz FL).- CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272).- Dual-Luciferase Reporter Assay for Pre-Screening CRISPR (d)Cas9-Mediated Epigenetic Editing on a Plant Promoter Using Human Cells.- In Silico Design of gRNA for CRISPR/Cas9-Mediated Gene Knock-Out.- CRISPR/Cas9 Vector Construction for Gene Knock-Out.- A Comprehensive Protocol for Assembly of Multiple gRNAs into a Direct Vector for Genome Editing in Tomato.- Genome Editing in Brassica juncea Using CRISPR/Cas9 Technology.- Precision Genome Editing with CRISPR-Cas9.- Using Magnetic Fields to Enhance the Seed Germination, Growth, and Yield of Plants.- Phenotypic and Genotypic Methods for the Identification and Quantification of Cyanobacteria in Lake Water.
Erscheinungsdatum | 27.04.2024 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 1 Illustrations, black and white; XIV, 413 p. 1 illus. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | Bioinformatics • Genome Analysis • Metabolomics • Plant Biology • Validation |
ISBN-10 | 1-0716-3781-9 / 1071637819 |
ISBN-13 | 978-1-0716-3781-4 / 9781071637814 |
Zustand | Neuware |
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