Protein Targeting Protocols

Surface Plasmon Resonance: Measuring Protein Interactions in Real Time G. Panayotou. Microcalorimetry of Protein-Protein Interactions A. Cooper. Partitioning of Proteins in Triton X-114 J. G. Pryde. Co-immunoprecipitation: Identification of Interacting Proteins N. G. Anderson. Overlay Ligand Blotting and Band-Shift Techniques to Study Kinase Anchoring Z. E. Hausken V. M. Coghlan and J. D. Scott. Size-Exclusion Chromatography: Identification of Interacting Proteins J. Beattie. Overlay and Bead Assay: Determination of Calcium Channel Subunit Interaction Domains V. E. S. Scott C. A. Gurnett and K. P. Campbell. Use of GST- Fusion and Related Constructs for the Identification of Interacting Proteins M. Harris. Molecular Genetic Approaches I: Two Hybrid Systems G. B. Bolger. Molecular Genetic Approaches II: Expression-Interaction Cloning M. Csukai and D. Mochly-Rosen. Molecular Genetic Approaches III: Determination of Protein Sequence Motifs Involved in Protein Targeting by Use of Coupled Transcription-Translation Systems G. Scotland and M. D. Houslay. Molecular Genetic Approaches IV: Recombinant Expression of Wild-Type and Acylation-Resistant G-Protein a-Subunits Using Transient Transfection Systems M. A. Grassie and G. Milligan. Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions J. D. Scott and M. C. Faux. SH2 and SH3 Domains: Unraveling Signaling Networks with Peptide Antagonists R. Stein. PH Domain of Serine-Threonine Protein Kinase B (RAC-PKB): Expression and Binding Assay for Phosphoinositides and Inositol Phosphates M. Frech and B. A. Hemmings. Membrane Targeting via Protein N-Myristoylation R. A. J. McIlhinney. Membrane Targeting via Protein Palmitoylation M. Veit and M. F. G. Schmidt. Agonist-Mediated Turnover of G-Protein a-Subunit Palmitoyl Groups: Role in Membrane Insertion M. A. Grassie and G. Milligan. Membrane Targeting via Protein Prenylation J. H. Overmeyer R. A. Erdman and W. A. Maltese. Nuclear Protein Import in a Permeabilized Cell Assay F. Melchior. Clathrin-Coated Vesicles and Targeting: Preparation of Adaptor Proteins A. Pauloin. Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway: A Subcellular Fractionation Approach E. Chanat A. S. Ditti' and S. A. Tooze. Index.