Plant Cell Culture

1. Isolation and maintenance of callus and cell suspension cultures ; 2. Isolation, culture, and regeneration of protoplasts ; 3. Applications of protoplast technology ; 3.A Fusion and selection of somatic hybrids ; 3.B Transient gene expression and stable transformation ; 3.C Studies with viruses ; 4. Selection of plant cells for desirable characteristics ; 4.A Inhibitor resistance ; 4.B Cold tolerance ; 4.C In vitro selection for salt tolerance ; 4.D In vitro selection for disease/toxin resistance ; 5. Plant regeneration via embryonic suspension cultures ; 6. Applied aspects of plant regeneration ; 6.A Micropropagation ; 6.B Virus-free plants ; 6.C Artificial seeds ; 7. Cryopreservation ; 8. Secondary products from cultured cells and organs I: molecular and cellular approaches ; 9. Secondary products from cultured cells and organs II: large scale culture