Plant Peptide Hormones and Growth Factors

Bioinformatics Methods for Prediction of Gene Families Encoding Extracellular Peptides.- Identification of Bioactive Phytocytokines Using Transcriptomic Data and Plant Bioassays.- Purification of Phytaspases Using a Biotinylated Peptide Inhibitor.- Characterization of Phytaspase Proteolytic Activity Using Fluorogenic Peptide Substrates.- Characterization of prolyl-4 hydroxylase substrate specificity using Pichia pastoris as an effective eukaryotic expression system.- Extraction of Apoplastic Peptides for the Structural Elucidation of Mature Peptide Hormones in Arabidopsis.- Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings.- A Seedling Growth Inhibition Assay to Measure Phytocytokine Activity.- A Trojan Horse Aproach Using Ustilago maydis to Study Apoplastic Maize (Zea mays) Peptides in situ.- Feeding Assay to Study the Effect of Phytocytokines on Direct and Indirect Defense in Maize.- A Quick Method to Analyze Peptide-Regulated Anthocyanin Biosynthesis.- Quantitative Measurement of Pattern-Triggered ROS Burst as an Early Immune Response in Tomato.- Automated Real-Time Monitoring of Extracellular pH to Assess Early Plant Defense Signaling.- Peptide-Mediated Cyclic Nucleotide Signaling in Plants: Identification and Characterization of Interactor Proteins with Nucleotide Cyclase Activity.- Detection of Ligand-Induced Receptor Kinase and Signaling Component Phosphorylation With Mn2+-Phos-Tag SDS-PAGE.- In-vivo Cross Linking of Biotinylated Peptide Ligands to Cell Surface Receptors.- Evaluation of Direct Ligand-Receptor Interactions by Photoaffinity Labeling.- Rapid Identification of Peptide-Receptor-Coreceptor Complexes in Protoplasts.- Acridinium-Based Chemiluminescent Receptor-Ligand Binding Assay for Protein/Peptide Hormones.- LuBiA (Luciferase-based Binding Assay): Glowing Peptides as Sensitive Probes to Study Ligand-Receptor Interactions.- Microscale Thermophoresis (MST) to Study Rapid Alkalinization Factor (RALF) - Receptor Interactions.- Isothermal Titration Calorimetry to Study Plant Peptide Ligand-Receptor Interactions.