Cilia
Springer-Verlag New York Inc.
978-1-0716-3506-3 (ISBN)
Cutting-edge and thorough, Cilia: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium.- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures.- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells.- Expansion Microscopy of Ciliary Proteins.- Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes.- Structural Analysis of Sperm Centrioles Using N-STORM.- A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium.- Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets.- Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging.- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells.- Proximity Mapping of Ciliary Proteins by BioID.- Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice using Endogenous Streptavidin/FLAG Tags.- Primary Human Nasal Epithelial Cell Culture.- BMI1 Transduction of Human Airway Epithelial Cells for Expansion of Proliferation and Differentiation.- High Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture Models.- Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods.- Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.
Erscheinungsdatum | 21.10.2023 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 50 Illustrations, color; 6 Illustrations, black and white; XII, 278 p. 56 illus., 50 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Ciliary proteins • Ciliopathies • homeostasis • human disease • tissue development • Toxicant exposure |
ISBN-10 | 1-0716-3506-9 / 1071635069 |
ISBN-13 | 978-1-0716-3506-3 / 9781071635063 |
Zustand | Neuware |
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