Plant Cytogenetics and Cytogenomics
Springer-Verlag New York Inc.
978-1-0716-3225-3 (ISBN)
Authoritative and practical, Plant Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal resource for plant scientists interested in molecular and evolutionary biology, breeding, systematics, and plant -omics in general.
Plant Cytogenetics: From Chromosomes to Cytogenomics.- The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants.- Nuclear DNA Content Estimation of Seaweed by Fluorimetry Analysis.- K-Mer-Based Genome Size Estimation in Theory and Practice.- A Bioinformatic Pipeline to Estimate Ploidy Level from Target Capture Sequence Data Obtained from Herbarium Specimens.- Nitrous Oxide-Induced Metaphase Arrest: A Technique for Somatic Chromosome Analysis.- Preparation of Mitotic Chromosomes with the Squash Technique.- Preparation of Mitotic Chromosomes with the Dropping Technique.- Laser Capture Microdissection: From Genomes to Chromosomes, from Complex Tissue to Single-Cell Analysis.- Flow Cytometric Analysis and Sorting of Plant Chromosomes.- C-Banding of Plant Chromosomes.- CMA/DAPI Banding of Plant Chromosomes.- Silver Nitrate Staining of Nucleolar Organizer Regions (Ag-NORs) in Plant Chromosomes.- Chromatin Immunostaining of Plant Nuclei.- Critical Steps in DAPI and FISH Imaging of Chromosome Spread Preparations.- Formamide-Free Genomic In Situ Hybridization (ff-GISH).- The Use of Ribosomal DNA for Comparative Cytogenetics.- Identification of the Sequence and the Length of Telomere DNA.- Chromosome Painting Using Chromosome-Specific BAC Clones.- CRISPR-FISH: A CRISPR/Cas9-Based In Situ Labeling Method.- Preparation of Male Meiotic Chromosomes for Fluorescence In Situ Hybridization and Immunodetection with Major Focus on Dogroses.- Extended DNA Fibers for High-Resolution Mapping.- Visualizing Chromosome Territories and Nuclear Architecture of Large Plant Genomes Using Alien Introgressions.- Visualization of the Nucleolus Using 5´Ethynyl Uridine.- Bioinformatics Prediction of Bulked Oligonucleotide Probes for FISH Using Chorus2.- Visualization of Oligonucleotide-Based Probes along Pseudochromosomes Using RIdeogram, KaryoploteR, and Circlize (Circos).- Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding.- Flow Sorting-Assisted Optical Mapping.- Chromosome Conformation Capture of Mitotic Chromosomes.- Analysis of 5S rDNA Genomic Organization through the RepeatExplorer2 Pipeline: A Simplified Protocol.- Tools for Drawing Informative Idiograms.- Using ChromEvol to Determine the Mode of Chromosomal Evolution.- Online Resources Useful for Plant Cytogenetics and Cytogenomics Research.
Erscheinungsdatum | 22.06.2023 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 106 Illustrations, color; 14 Illustrations, black and white; XVIII, 568 p. 120 illus., 106 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-0716-3225-6 / 1071632256 |
ISBN-13 | 978-1-0716-3225-3 / 9781071632253 |
Zustand | Neuware |
Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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