Cell Polarity Signaling

Use of Fluorescence Recovery after Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins.- FRET Imaging of Rho GTPase Activity with Red Fluorescent Protein-Based FRET Pairs.- Live‐Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics.- Single-Cell Single Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates.- Biochemical Assays to Detect Activation of Small GTPases Rho, Rac, and Cdc42 during Morphogenesis.- Analysis of Planar Cell Polarity Complexes by Proximity Biotinylation in Xenopus Embryos.- Regulation of Cell Polarity by Posttranslational Protein Palmitoylation.- Enrichment and Detection of Wnt Proteins from Cell Culture Media.- Using Live-Imaging to Examine Early Cardiac Development in Zebrafish.- Imaging Planar Cell Polarity Proteins in Xenopus Neuroectoderm.- In Ovo Gain- and Loss-of-Function Approaches to Study Gut Morphogenesis.- Avian Embryosas a Model to Study Vascular Development.- BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice.- Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics.- Protocols for Investigating the Epithelial Properties of Cardiac Progenitor Cells in the Mouse Embryo.- Methods to Investigate Cell Polarity of Inner Ear.- Characterization of Axon Guidance Phenotypes in Wnt/PCP Mutant Mice.- In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.- Biochemical and Cellular Assays to Study Mechanisms of PCP Signaling in Axon Guidance.- Dissection, Fixation, and Immunostaining of the Drosophila Midgut.- In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands.- Imaging Epidermal Cell Rearrangement in the C. elegans Embryo.- Methods for the Study of Apical Constriction during Ascidian Gastrulation.- Assays for Apical Constriction Using the Xenopus Model.- The Use of 3-Dimensional Cell Culture to Study Apicobasal Polarization and Lumen Formation.- Studying Cell Polarity Dynamics during Cancer Initiation Using Inducible 3D Organotypic Cultures.- Under-Agarose Chemotaxis and Migration Assays for Dictyostelium.- Mapping Asymmetry in Collective Cell Migration: Lessons from Border Cells in Drosophila Oogenesis.- Targeted Injections, Atomic Force Microscopy, and Hydrogels: A Toolbox to Study Tissue Mechanics In Vivo and Ex Vivo.- Quantitative Analysis of Directional Neural Crest Cell Migration.