Peroxisomes

Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation.- Analysis of Yeast Peroxisomes via Spatial Proteomics.- Isolation of Glycosomes from Trypanosoma brucei.- Immunolabeling for Detection of Endogenous and Overexpressed Peroxisomal Proteins in Mammalian Cells.- Super-Resolution Imaging of Peroxisomal Proteins Using STED Nanoscopy.- Direct Stochastic Optical Reconstruction Microscopy (dSTORM) of Peroxisomes.- Correlative Light- and Electron Microscopy in Peroxisome Research.- Ultrastructural Analysis and Quantification of Peroxisome-Organelle Contacts.- Detection of Peroxisomal Proteins during Mycobacterial Infection.- Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction.- Assay of Reactive Oxygen/Nitrogen Species (ROS/RNS) in Arabidopsis Peroxisomes through Fluorescent Protein Containing a Type 1-Peroxisomal Targeting Signal (PTS1).- Identification of Peroxisome-Derived Hydrogen Peroxide-Sensitive Target Proteins Using a YAP1C-Based GeneticProbe.- Assessment of the Peroxisomal Redox State in Living Cells Using NADPH- and NAD+/NADH-Specific Fluorescent Protein Sensors.- Live-Cell Imaging of Peroxisomal Calcium Levels and Dynamics.- Analysis of Peroxisome Biogenesis by Phos-Tag SDS-PAGE.- Targeted Modifications of the Yeast Genome to Study Peroxisomes.- Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions.- Generation of Reporter Cell Lines for Endogenous Expression Analysis of Peroxisomal Proteins.- Peroxisomes and Viruses: Overview on Current Knowledge and Experimental Approaches.- Tools to Investigate the Peroxisome-Dependent Antiviral Response.- Determining the Importance of Peroxisomal Proteins for Viral Infections in Cultured Mammalian Cells.- Characterization of Yeast Peroxisomes: Enrichment of Peroxisomal Fractions and Analysis of b-Oxidation Activity.- A Cell-Free In Vitro Import System for Peroxisomal Proteins Containing a Type 2 Targeting Signal (PTS2).- Assessing Peroxisomal Protein Interaction by Immunoprecipitation.- Purification of a Recombinant Human PEX1/PEX6 AAA+ ATPase Complex from HEK293TT Cells.- Affinity Purification of Soluble and Membrane-Bound Protein Complexes by a FlpIn Strategy.- Utilization of Nonstop mRNA to Assess Ribosome-Associated Nascent Polypeptide Chains in Early Topogenesis of Peroxisomal Proteins.- Computational Evaluation of Peroxisomal Targeting Signals in Metazoa.- Computational Approaches for Peroxisomal Protein Localization.- Estimating the Interaction Strength between PTS1-Peptides and Their Receptor PEX5 in Living Cells Using Flow-Cytometer-Based FRET (flowFRET) Measurements.- Using the Super Folder GFP (sfGFP) System to Study Plant Peroxisomal Protein Import.- Biochemical Fractionation of Trypanosomes for the Analysis of Glycosomal Protein Import Defects.- Manipulation and Visualization of Peroxisomes in Drosophila.- Mouse Models to Study Peroxisomal Functions and Disorders: Overview,Caveats, and Recommendations.