Fluorescent Proteins
Springer-Verlag New York Inc.
978-1-0716-2666-5 (ISBN)
Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.
Using FPbase: The Fluorescent Protein Database.- Selecting the Fluorescent Protein for In Vivo Imaging Experiments.- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo.- Directed Evolution of Fluorescent Proteins in Bacteria.- Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation.- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization.- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization.- CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line.- Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants.- Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions.- DNA Visualization Using Fluorescent Proteins.- Quantification ofIntracellular Citrate Concentrations with Genetically Encoded Biosensors.- Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out.- Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae.- Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells.- Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins.- Determining Protein-Protein Interaction with GFP-Trap Beads.
Erscheinungsdatum | 10.10.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2564 |
Zusatzinfo | 80 Illustrations, color; 15 Illustrations, black and white; XI, 326 p. 95 illus., 80 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Naturwissenschaften ► Chemie | |
Schlagworte | Biological Systems • biosensors • Cell biological applications • FP dynamics • Protein function and behavior |
ISBN-10 | 1-0716-2666-3 / 1071626663 |
ISBN-13 | 978-1-0716-2666-5 / 9781071626665 |
Zustand | Neuware |
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