Candida auris
Springer-Verlag New York Inc.
978-1-0716-2416-6 (ISBN)
Cutting-edge and practical, Candida auris: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field.
Isolation of Candida auris in Clinical Specimens .- Candida auris Identification and Profiling by MALDI-ToF Mass Spectrometry.- Molecular Tools for Candida auris Identification and Typing.- qPCR Detection of Candida auris using the GPS™ CanAur MONODOSE dtec-qPCR Test.- Susceptibility Testing of Candida auris Isolates.- Deciphering Candida auris Paradoxical Growth Effect (Eagle Effect) in Response to Echinocandins.- An Optimized Electroporation Procedure for Genetic Transformation of Candida auris.- Genetic Transformation of Candida auris via Homology-Directed Repair Using a Standard Lithium-Acetate Protocol.- Downregulation of Essential Genes in the Fungal Pathogen Candida auris.- Cell Viability Assays for Candida auris.- Measuring Gene Expression via mRNA Abundance in Candida auris.- Gas Chromatography–Mass Spectrometry (GC-MS) Analysis of Candida auris Metabolites.- Isolation of Extracellular Vesicles from Candida auris.- Labelling of Candida auris Cell Walls to Examine Levels of PAMP Exposure by Flow Cytometry and Fluorescence Microscopy.- High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall.- Generating Complete Genome Assemblies of Candida auris.- MycoSNP: A Portable Workflow for Performing Whole-Genome Sequencing Analysis of Candida auris.- Bioinformatic Identification of ABC Transporters in Candida auris.- Examining Neutrophil-Candida auris Interactions with Human Neutrophils Ex Vivo.- Modeling Candida auris Colonization on Porcine Skin Ex Vivo.- A Human Ex Vivo Skin Model to Study Candida auris Biofilms.- In Vivo Skin Colonization/Decolonization Models for Candida auris.- Use of Galleria mellonella Larvae to Study the Pathogenicity and Clonal Lineage-Specific Behaviors of the Emerging Fungal Pathogen Candida auris.- Drosophila melanogaster as a Rapid and Reliable In Vivo Infection Model to Study the Emerging Yeast Pathogen Candida auris.- An Immunocompromised Mouse Model of Candida auris Systemic Infection.- Mouse Gastrointestinal Colonization Model for Candida auris.
Erscheinungsdatum | 15.06.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2517 |
Zusatzinfo | 60 Illustrations, color; 16 Illustrations, black and white; XV, 342 p. 76 illus., 60 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie |
Naturwissenschaften ► Biologie ► Mykologie | |
Schlagworte | cell viability assays • Echinocandins • Fungal Predators • Hsp90 • MALDI-ToF MS |
ISBN-10 | 1-0716-2416-4 / 1071624164 |
ISBN-13 | 978-1-0716-2416-6 / 9781071624166 |
Zustand | Neuware |
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